cDNA
MGC:63577
- ID
- ZDB-CDNA-040425-2104
- Name
- MGC:63577
- Symbol
- MGC:63577
- Previous Names
-
- FR_5273 (1)
- Type
- cDNA
- Location
- Unmapped
- Genome Resources
- None
- Species
- Danio rerio
- Library
- Sugano SJD adult male
- Digest
- Insert Size
- 1648
- Cloning Site
- EcoRI-PstI
- Vector Type
- Plasmid
- Vector
- pME18S-FL3
- Polymerase
- T3 RNA polymerase
- PCR Amplification
-
Reaction denatured 4 min. followed by PCR cycling 95°C 30s, 55°C 30s, 72°C 3 min. (at least 1 min. per kb) followed by elongation at 72°C 7 min.
5' TGTACGGAAGTGTTACTTCTGCTC3'
5'GGATCCATTAACCCTCACTAAAGGGAAGGCCGCGACCTGCAGCTC3' - Suppliers
- None
- Quality
- ( Probe is difficult to use. Generally basal level of expression with more intense labeling in particular structure. )
- Protocol
- Thisse in situ hybridization protocol
- Note
- None
Gene Expression
- Directly Submitted Expression Data
- 6 figures (5 images) from Thisse et al., 2004 [MGC:63577]
Marker Relationships
Sequences
Citations