MGC:77581

ID

ZDB-CDNA-040425-159

Name

MGC:77581

Symbol

MGC:77581

Previous Names

None

Type

cDNA

Location

Unmapped

Genome Resources

None

Species

Danio rerio

Library

NIH_ZGC_10

Digest

Insert Size

1727

Cloning Site

Sfi A - Sfi B

Vector Type

Plasmid

Vector

pExpress-1

Polymerase

T3 RNA polymerase

PCR Amplification

Reaction denatured 4 min. followed by PCR cycling 95°C 30s, 55°C 30s, 72°C 3 min. (at least 1 min. per kb) followed by elongation at 72°C 7 min.
5' TGG ATA ACC GTA TTA CCG CC 3'
5' CGC GCA ATT AAC CCT CAC TAA AGC ACT AGT CAT ACC AGG ATC 3'

Suppliers

None

Quality

( Simple to use, intense expression pattern restricted to a few structures )

Protocol

Thisse in situ hybridization protocol

Note

None