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Measurement Methods Ontology

nitrite analysis using the Griess reaction

Term ID
MMO:0000370
Synonyms
  • Griess test
  • Griess test for nitric oxide
  • nitric oxide analysis using the Griess reaction
  • nitrite analysis using the Griess reagent
Definition
Method of assessing the amount of nitrite, the nitrogen oxoanion chemical formula NO2(-), in a solution by treating with a diazotizing reagent, e.g. sulfanilamide (SA), in acidic media to form a transient diazonium salt which is then allowed to react with a coupling reagent, N-naphthyl-ethylenediamine (NED), to form a stable azo compound with an intense purple color that absorbs light at 540 nm. Measurement of nitrite is used to estimate nitric oxide (NO) production in biological systems.
References
Ontology
Measurement Methods Ontology
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