ferric uptake regulator titration assay evidence

Term ID

ECO:0001829

Synonyms

• Fluorescence polarization
• FURTA

Definition

A type of reporter gene assay evidence used to locate and isolate Fur sites where a plasmid library is created and cloned into a cell line with a ferric uptake regulator (Fur)-repressed reporter and the Fur protein is titrated away from its binding site on the reporter, after which the cell is isolated and plasmid extracted for further sequence analysis. (3)

References

Ontology

Evidence Ontology