rapid amplification of cDNA ends polymerase chain reaction evidence

Term ID

ECO:0001820

Synonyms

• 3? RACE-PCR
• 5? RACE-PCR
• RACE PCR

Definition

A type of nucleotide sequencing assay evidence in which RT-PCR (cDNA synthesis) is first used to produce a cDNA copy of a region of the RNA transcript being investigated followed by PCR to capture either the unknown 5' or 3' end of the transcript for sequencing, depending on whether 5' RACE-PCR or 3' RACE-PCR are being undertaken. (2)

References

Ontology

Evidence Ontology