bait-prey hybrid interaction evidence

Term ID

ECO:0000025

Synonyms

• bait-prey protein pull-down evidence

Definition

A type of protein binding evidence where proteins of interest (bait and prey) are covalently linked to incomplete fragments of a third protein (reporter) and expressed in vivo, at which time interaction between bait and prey proteins brings reporter fragments in close enough proximity to allow them to reform and become a functional reporter protein.

References

• MI:0090

Ontology

Evidence Ontology