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Mercatali et al., 2016 - Development of a Patient-Derived Xenograft (PDX) of Breast Cancer Bone Metastasis in a Zebrafish Model. International Journal of Molecular Sciences   17(8) Full text @ Int. J. Mol. Sci.

Fig. 2

Representative image of MDA-MB-231 cell line five days after injection into the duct of Couvier of 2 day post fertilization (dpf), Tg(fli1:GFP) ZF embryo. MDA-MB-231, labeled in red, were monitored on a daily basis for the duration of the experiment (five days) and showed progressive and extensive dissemination throughout the developing embryo (25× magnification).

EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Term:
Stage: Long-pec

Fig. 3

Images of carboxyfluorescein succinimidyl ester (CFSE)-labeled, patient-derived breast cancer bone metastasis (primary cells) xenografted in Tg(kdrl:mcherry) ZF embryos. (A) Whole-body image of the zebrafish embryo at 5 dpi (25× magnification). Primary cells disseminated predominantly in the caudal hematopoietic tissues (CHT) of the embryo; (B) Details of (A), showing the interactions of primary cells with the zebrafish vessels in the CHT. Cells extravasated in the CHT of the ZF embryo and engrafted in close proximity of the vessels. Fluorescent images merged with brightfield image, 63× magnification; (C) Combined picture of the CHT of embryo in (A,B). Individual images were taken at 63× magnification.

EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Term:
Stage: Days 7-13
Acknowledgments:
ZFIN wishes to thank the journal International Journal of Molecular Sciences for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Int. J. Mol. Sci.