
1. spermatogonium A (SgA)


2. early spermatogonium B (eSgB)



3. early spermatogonium B (eSgB)




4. late spermatogonium B



5. late spermatogonium B: clusters >4 cells


6. spermatocyte, leptotene stage of meiotic prophase ("threadlike" appearance of chromosomes).

7. spermatocyte, leptotene stage of meiotic prophase, progressing to the zygotene stage; Note that there is staining with Putt's carbol fuchsin from this stage on, although still faint here..

8. spermatocyte, zygotene stage of meiotic prophase ("bouquet" configuration of chromosomes).

9. spermatocyte, zygotene stage of meiotic prophase ("umbrella" configuration of chromosomes).

10. pachytene primary spermatocytes; in this stage, there is longitudinal contraction of the chromosomes, and the chromatin strands have a coarse appearance.

11. sdividing primary spermatocytes (diplotene stage); the metaphase, anaphase, and telophase of the first meiotic division (respectively equatorial arrangement of chromosomes, movement to cell poles, and actual division of the cell) are completed quickly, and are therefore only present briefly. The daughter cells of the first meiotic division are known as secundary spermatocytes (diploid). The second meiotic division is also completed quickly, and therefore secundary spermocytes are neither easily discernible. The resulting cells of the second meiotic division are:

12. spermatids (haploid).

13. spermatids.

14. mature spermatozoa are released from open spermatocysts into the lumen of the tubules (spermiation).
Reference (for Putt's carbol fuchsin)
- Berg-JW. Differential staining of spermatozoa in sections of testis. Am.J.Clin.Pathol.23:513-515;1953.