TALEN

TALEN1-lrp4

ID

ZDB-TALEN-151216-1

Name

TALEN1-lrp4

Previous Names

None

Target

lrp4 (1)

Target Sequence 1

5' - TCCTCCATGTGCGCCCGAT - 3'

Target Sequence 2

5' - ATGGCCGCTGTATTGGACA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

According to the authors, the lrp4 region is complicated because it is partially annotated in GRCz10, but the sequences targeted with the TALENs are not included in the annotation. The targeted sequence was previously annotated in Ensembl's Zv9 assembly from which the TALENs were designed. Sequencing of lrp4 cDNA produces sequence that BLASTs to this unannotated region of chromosome 7, supporting the idea that this TALEN pair does target lrp4.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
p184	lrp4
p185	lrp4

Expression

Gene expression in Wild Types + TALEN1-lrp4

No data available

Phenotype

Phenotype resulting from TALEN1-lrp4

No data available

Phenotype of all Fish created by or utilizing TALEN1-lrp4

Phenotype	Fish	Conditions	Figures
muscle acetylcholine-gated channel complex decreased amount, abnormal	lrp4^p184/p184	standard conditions	Fig. 6 from Remédio et al., 2016
establishment of synaptic specificity at neuromuscular junction decreased occurrence, abnormal	lrp4^p184/p184	standard conditions	Fig. 6 from Remédio et al., 2016
motor neuron regeneration process quality, abnormal	lrp4^p184/p184; ml2Tg	transection: motor neuron	Fig. 1 , Fig. 2 from Gribble et al., 2018
neuromuscular junction development decreased occurrence, abnormal	lrp4^p184/p184; ml2Tg	control	Fig. 5 from Gribble et al., 2018
motor neuron regeneration decreased efficacy, abnormal	lrp4^p184/p184; ml2Tg	transection: motor neuron	Fig. 1 , Fig. 2 from Gribble et al., 2018
skeletal muscle cell neuromuscular junction znp-1 labeling spatial pattern, abnormal	lrp4^p184/p184; ml2Tg	control	Fig. 5 from Gribble et al., 2018
motor neuron axon extension involved in regeneration process quality, abnormal	lrp4^p184/p184; ml2Tg	transection: motor neuron	Fig. 2 from Gribble et al., 2018
motor neuron axon extension involved in regeneration process quality, abnormal	lrp4^p184/p184; ml2Tg; p169Tg	transection: motor neuron	Fig. 2 from Gribble et al., 2018
myelinating Schwann cell morphology, abnormal	lrp4^p184/p184; ml2Tg; p169Tg	transection: motor neuron	Fig. 3 from Gribble et al., 2018
motor neuron regeneration decreased efficacy, abnormal	lrp4^p184/p184; ml2Tg; p169Tg	transection: motor neuron	Fig. 2 from Gribble et al., 2018
motor neuron regeneration process quality, abnormal	lrp4^p184/p184; ml2Tg; p169Tg	transection: motor neuron	Fig. 2 from Gribble et al., 2018

Citations