Term Name: ferric uptake regulator titration assay evidence
Synonyms: Fluorescence polarization, FURTA
Definition: A type of reporter gene assay evidence used to locate and isolate Fur sites where a plasmid library is created and cloned into a cell line with a ferric uptake regulator (Fur)-repressed reporter and the Fur protein is titrated away from its binding site on the reporter, after which the cell is isolated and plasmid extracted for further sequence analysis.
Ontology: Evidence Ontology [ECO:0001829]

Relationships
is a type of: reporter gene assay evidence
has subtype: ferric uptake regulator titration assay evidence used in automatic assertion ferric uptake regulator titration assay evidence used in manual assertion