| Term Name: | ferric uptake regulator titration assay evidence |
|---|---|
| Synonyms: | Fluorescence polarization, FURTA |
| Definition: | A type of reporter gene assay evidence used to locate and isolate Fur sites where a plasmid library is created and cloned into a cell line with a ferric uptake regulator (Fur)-repressed reporter and the Fur protein is titrated away from its binding site on the reporter, after which the cell is isolated and plasmid extracted for further sequence analysis. |
| Ontology: | Evidence Ontology [ECO:0001829] |