Term Name:	chain termination sequencing evidence
Synonyms:	dye terminator sequencing, Sanger sequencing
Definition:	A type of nucleotide sequencing assay in which DNA sequence is determined by addition of a primed DNA template to a reaction mixture, followed by addition of DNA polymerase, deoxynucleosidetriphosphates (dNTPs - one of which may be radiolabeled), and one of four di-deoxynucleotidetriphosphates (ddNTPs), after which DNA elongation is terminated and the DNA is separated by size and imaged.
Ontology:	Evidence Ontology [ECO:0000225]