Term Name: manual assertion
Synonyms:
Definition: An assertion method that involves human review.
Ontology: Evidence Ontology [ECO:0000218]

Relationships
is a type of: assertion method
inverse disjoint_from: automatic assertion
inverse union_of: assertion method
inverse used_in: 3C-qPCR evidence used in manual assertion 3C-seq evidence used in manual assertion 3D cell culture evidence used in manual assertion 4',6-diamidino-2-phenylindole staining evidence used in manual assertion 4C evidence used in manual assertion 5-cyano-2,3-ditolyl tetrazolium chloride staining evidence used in manual assertion 5C evidence used in manual assertion 7-aminoactinomycin staining evidence used in manual assertion 51Cr release assay evidence used in manual assertion 454 pyrosequencing evidence used in manual assertion [3H]-thymidine incorporation assay evidence used in manual assertion [3H]arachidonic acid release assay evidence used in manual assertion ad-hoc qualitative phenotype observation evidence used in manual assertion ad-hoc quantitative phenotype observation evidence used in manual assertion adhesion assay evidence used in manual assertion adoptive cell transfer evidence used in manual assertion affinity chromatography evidence used in manual assertion affinity evidence used in manual assertion Affymetrix GeneChip evidence used in manual assertion agglutination test evidence used in manual assertion air-liquid interface assay evidence used in manual assertion alamarBlue assay evidence used in manual assertion alkaline phosphatase reporter gene assay evidence used in manual assertion allograft transplantation phenotypic evidence used in manual assertion analytical ultracentrifugation evidence used in manual assertion animal model system study evidence used in manual assertion anion-exchange chromatography evidence used in manual assertion annexin-V staining evidence used in manual assertion anti-sense experiment evidence used in manual assertion antibody 4B7/8-specificity based MHC presentation evidence used in manual assertion antibody 10.3.6-specificity based MHC presentation evidence used in manual assertion antibody 14.4.4-specificity based MHC presentation evidence used in manual assertion antibody 28-14-8S-specificity based MHC presentation evidence used in manual assertion antibody 34-1-2S-specificity based MHC presentation evidence used in manual assertion antibody 34-5-8S-specificity based MHC presentation evidence used in manual assertion antibody 34-7-23S-specificity based MHC presentation evidence used in manual assertion antibody AG2.42.7-specificity based MHC presentation evidence used in manual assertion antibody B7/21-specificity based MHC presentation evidence used in manual assertion antibody B8-24-3-specificity based MHC presentation evidence used in manual assertion antibody B8.11.2-specificity based MHC presentation evidence used in manual assertion antibody B22-249.R1-specificity based MHC presentation evidence used in manual assertion antibody B123.2-specificity based MHC presentation evidence used in manual assertion antibody BB7.2-specificity based MHC presentation evidence used in manual assertion antibody C7709A2-specificity based MHC presentation evidence used in manual assertion antibody CR11-351-specificity based MHC presentation evidence used in manual assertion antibody DK1G8-specificity based MHC presentation evidence used in manual assertion antibody DT9-specificity based MHC presentation evidence used in manual assertion antibody HB-164-specificity based MHC presentation evidence used in manual assertion antibody HB145-specificity based MHC presentation evidence used in manual assertion antibody HB180-specificity based MHC presentation evidence used in manual assertion antibody ILA-21-specificity based MHC presentation evidence used in manual assertion antibody ILA-88-specificity based MHC presentation evidence used in manual assertion antibody IVD12-specificity based MHC presentation evidence used in manual assertion antibody L243-specificity based MHC presentation evidence used in manual assertion antibody LB3.1-specificity based MHC presentation evidence used in manual assertion antibody M1/42-specificity based MHC presentation evidence used in manual assertion antibody M5/114-specificity based MHC presentation evidence used in manual assertion antibody ME1-specificity based MHC presentation evidence used in manual assertion antibody MKD6-specificity based MHC presentation evidence used in manual assertion antibody OX18-specificity based MHC presentation evidence used in manual assertion antibody PT85-A-specificity based MHC presentation evidence used in manual assertion antibody SF1-1.1.1-specificity based MHC presentation evidence used in manual assertion antibody SPVL3-specificity based MHC presentation evidence used in manual assertion antibody Tu36-specificity based MHC presentation evidence used in manual assertion antibody Tu39-specificity based MHC presentation evidence used in manual assertion antibody W6/32-specificity based MHC presentation evidence used in manual assertion antibody Y-3-specificity based MHC presentation evidence used in manual assertion antibody Y3JP-specificity based MHC presentation evidence used in manual assertion antibody-specificity based MHC presentation evidence used in manual assertion apoptotic assay evidence used in manual assertion array-based sequence capture evidence used in manual assertion asexual sporulation assay evidence used in manual assertion atomic force microscopy evidence used in manual assertion ATP bioluminescence assay evidence used in manual assertion author inference based on intrinsic disorder prediction used in manual assertion author inference used in manual assertion author statement supported by traceable reference used in manual assertion author statement supported from referenced clinical study used in manual assertion author statement used in manual assertion author statement without traceable support used in manual assertion automatically integrated combinatorial computational and experimental evidence used in manual assertion automatically integrated combinatorial computational evidence used in manual assertion automatically integrated combinatorial evidence used in manual assertion automatically integrated combinatorial experimental evidence used in manual assertion bacterial 2-hybrid assay evidence used in manual assertion bacterial one-hybrid evidence used in manual assertion bait-prey hybrid interaction evidence used in manual assertion bait-prey interaction evidence used in manual assertion bait-prey protein pull-down evidence used in manual assertion beta galactosidase functional complementation evidence used in manual assertion beta-galactosidase fusion protein localization evidence used in manual assertion beta-galactosidase protein immunolocalization evidence used in manual assertion beta-galactosidase reporter gene assay evidence used in manual assertion beta-glucuronidase fusion protein localization evidence used in manual assertion beta-glucuronidase reporter gene assay evidence used in manual assertion bio-layer interferometry evidence used in manual assertion biochemical trait analysis evidence used in manual assertion biofilm formation assay evidence used in manual assertion biological aspect of ancestor evidence used in manual assertion biological aspect of descendant evidence used in manual assertion biological system reconstruction evidence based on homology evidence used in manual assertion biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion biological system reconstruction evidence based on orthology evidence used in manual assertion biological system reconstruction evidence based on paralogy evidence used in manual assertion biological system reconstruction evidence by experimental evidence from mixed species used in manual assertion biological system reconstruction evidence by experimental evidence from single species used in manual assertion biological system reconstruction evidence by experimental evidence used in manual assertion biological system reconstruction evidence used in manual assertion biological target-disease association via drug evidence used in manual assertion BLAST evidence used in manual assertion blocking monoclonal antibody evidence used in manual assertion blocking peptide evidence used in manual assertion blocking polyclonal antibody evidence used in manual assertion blood test evidence used in manual assertion Boyden chamber assay evidence used in manual assertion bromodeoxyuridine incorporation assay evidence used in manual assertion cap analysis of gene expression evidence used in manual assertion capillary assay evidence used in manual assertion carboxyfluorescein diacetate succinimidyl ester staining evidence used in manual assertion caspase assay evidence used in manual assertion cDNA to DNA expression microarray evidence used in manual assertion cell aggregation evidence used in manual assertion cell counting evidence used in manual assertion cell fractionation evidence used in manual assertion cell growth assay evidence used in manual assertion cell growth regulation assay evidence used in manual assertion cell permeability assay evidence used in manual assertion cell population optical density evidence used in manual assertion cell proliferation assay evidence used in manual assertion cell staining evidence used in manual assertion cell transfection experiment evidence used in manual assertion cell viability assay evidence used in manual assertion cell-attached single-channel recording evidence used in manual assertion cell-based assay evidence used in manual assertion cell-detached inside-out single-channel recording evidence used in manual assertion cell-detached outside-out single-channel recording evidence used in manual assertion cell-free assay evidence used in manual assertion chain termination sequencing evidence used in manual assertion chemiluminescence-linked immunoassay evidence used in manual assertion chemotaxis assay evidence used in manual assertion chimeric protein phenotypic evidence used in manual assertion chloramphenicol acetyltransferase reporter gene assay evidence used in manual assertion chromatin immunoprecipitation- exonuclease evidence used in manual assertion chromatin immunoprecipitation-chip evidence used in manual assertion chromatin immunoprecipitation-PCR evidence used in manual assertion chromatin immunoprecipitation-qPCR evidence used in manual assertion chromatin immunoprecipitation-seq evidence used in manual assertion chromatography evidence used in manual assertion circular dichroism evidence used in manual assertion clinical study evidence used in manual assertion co-electrophoresis evidence used in manual assertion co-fractionation evidence used in manual assertion co-immunoprecipitation evidence used in manual assertion co-localization evidence used in manual assertion co-purification evidence used in manual assertion co-sedimentation assay evidence used in manual assertion coding sequence splice pattern evidence used in manual assertion cognitive assay phenotypic evidence used in manual assertion colony biofilm assay evidence used in manual assertion colony boundary assay evidence used in manual assertion colony color phenotypic evidence used in manual assertion colony counting evidence used in manual assertion colony diameter phenotype evidence used in manual assertion colony morphology phenotypic evidence used in manual assertion colony papillation assay phenotypic evidence used in manual assertion colony size phenotypic evidence used in manual assertion colorimetric in situ hybridization evidence used in manual assertion combinatorial computational evidence used in manual assertion combinatorial evidence used in manual assertion combinatorial experimental and author inference evidence contained in single publication used in manual assertion combinatorial experimental and author inference evidence used in manual assertion combinatorial experimental and curator inference evidence used in manual assertion comet assay evidence used in manual assertion comparative genomics motif search evidence used in manual assertion compement fixation assay evidence used in manual assertion competitive binding evidence used in manual assertion competitive growth assay evidence used in manual assertion compositional similarity evidence used in manual assertion computational evidence used in manual assertion computational inference used in manual assertion computational structure modeling evidence used in manual assertion conditional knockin evidence used in manual assertion conditional knockout evidence used in manual assertion confocal laser scanning microscopy evidence used in manual assertion confocal microscopy evidence used in manual assertion Congo red birefringence evidence used in manual assertion Congo red staining evidence used in manual assertion consensus search evidence used in manual assertion constitutively active mutant protein evidence used in manual assertion coordinate mapping to geographic property shapefiles used in manual assertion copper transport assay evidence used in manual assertion copper-phenanthroline footprinting evidence used in manual assertion cRNA to DNA expression microarray evidence used in manual assertion cross-linking evidence used in manual assertion cross-streak test evidence used in manual assertion cryogenic electron microscopy evidence used in manual assertion cryogenic electron microscopy-based structural model with high relative B-factor values used in manual assertion cryogenic electron microscopy-based structural model with missing residue coordinates used in manual assertion crystal violet staining evidence used in manual assertion crystallography evidence used in manual assertion curator inference based on intrinsic disorder prediction used in manual assertion curator inference from authoritative source used in manual assertion curator inference from book used in manual assertion curator inference from database used in manual assertion curator inference from dictionary used in manual assertion curator inference from encyclopedia used in manual assertion curator inference from journal publication used in manual assertion curator inference from MedlinePlus dictionary used in manual assertion curator inference from MedlinePlus encyclopedia used in manual assertion curator inference from Merriam-Webster Dictionary used in manual assertion curator inference from Oxford Dictionary used in manual assertion curator inference from Wikipedia used in manual assertion curator inference used in manual assertion current clamp recording evidence used in manual assertion current density evidence used in manual assertion cut-open oocyte voltage clamp recording evidence used in manual assertion Cya fusion reporter assay evidence used in manual assertion cytochalasin experiment evidence used in manual assertion cytochemistry evidence used in manual assertion cytochrome C release assay evidence used in manual assertion cytotoxicity assay evidence used in manual assertion deep learning neural network method evidence used in manual assertion deglycosylation assay evidence used in manual assertion deletion mutation phenotypic evidence used in manual assertion developmental similarity evidence used in manual assertion differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact) used in manual assertion differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact) used in manual assertion differential hybridization evidence used in manual assertion differential interference contrast microscopy evidence used in manual assertion differential methylation hybridization evidence used in manual assertion differential scanning calorimetry evidence used in manual assertion differential scanning fluorimetry evidence used in manual assertion direct assay evidence used in manual assertion direct Coombs test evidence used in manual assertion direct hemagglutination assay evidence used in manual assertion disk diffusion test evidence used in manual assertion DNA adenine methyltransferase identification evidence used in manual assertion DNA affinity chromatography evidence used in manual assertion DNA dot blot assay evidence used in manual assertion DNA laddering assay evidence used in manual assertion DNA synthesis cell proliferation assay evidence used in manual assertion DNA to cDNA expression microarray evidence used in manual assertion DNAse footprinting evidence used in manual assertion documented statement evidence used in manual assertion dominant-negative mutant phenotypic evidence used in manual assertion double mutant phenotypic evidence used in manual assertion duplication mutation evidence used in manual assertion dynamic fluorescence quenching evidence used in manual assertion dynamic light scattering assay evidence used in manual assertion ectopic expression evidence used in manual assertion Edman degradation evidence used in manual assertion electroencephalography recording evidence used in manual assertion electron microscopy evidence used in manual assertion electron paramagnetic resonance evidence used in manual assertion electrophoretic mobility shift assay evidence used in manual assertion electrophysiology assay evidence used in manual assertion electrospray ionization fourier transform ion cyclotron resonance mass spectrometry evidence used in manual assertion environmental perturbation phenotypic evidence used in manual assertion enzymatic activity assay evidence used in manual assertion enzyme inhibition evidence used in manual assertion enzyme-linked immunoabsorbent assay evidence used in manual assertion epifluorescence microscopy evidence used in manual assertion epistatic interaction phenotypic evidence used in manual assertion epitope-tagged protein immunolocalization evidence used in manual assertion eTag assay evidence used in manual assertion Etest evidence used in manual assertion evidence used in manual assertion experimental evidence used in manual assertion experimental phenotypic evidence used in manual assertion experimentally evolved mutant phenotypic evidence used in manual assertion expression library screen evidence used in manual assertion expression microarray evidence used in manual assertion expression pattern evidence used in manual assertion extended field laser confocal microscopy evidence used in manual assertion extracellular recording evidence used in manual assertion far-UV circular dichroism evidence used in manual assertion far-Western blotting evidence used in manual assertion ferric uptake regulator titration assay evidence used in manual assertion field potential recording evidence used in manual assertion filter binding assay evidence used in manual assertion flow cell biofilm assay evidence used in manual assertion flow cytometry evidence used in manual assertion fluorescence anisotropy evidence used in manual assertion fluorescence correlation spectroscopy evidence used in manual assertion fluorescence evidence used in manual assertion fluorescence in situ hybridization evidence used in manual assertion fluorescence microscopy evidence used in manual assertion fluorescence polarization evidence used in manual assertion fluorescence quantification assay evidence used in manual assertion fluorescence recovery after photobleaching evidence used in manual assertion fluorescence resonance energy transfer evidence used in manual assertion fluorescent sensor evidence used in manual assertion Fourier-transform infrared spectroscopy evidence used in manual assertion fractionation evidence used in manual assertion frameshift mutation phenotypic evidence used in manual assertion full support of intron positions by RNA-sequencing alignment evidence used in manual assertion functional complementation evidence used in manual assertion functional complementation in heterologous system evidence used in manual assertion gain-of-function mutant phenotypic evidence used in manual assertion GAL4-VP16 functional complementation evidence used in manual assertion galactokinase reporter gene assay evidence used in manual assertion gel-filtration evidence used in manual assertion gene expression similarity evidence used in manual assertion gene neighbors evidence used in manual assertion genetic interaction evidence used in manual assertion genetic similarity evidence used in manual assertion genetic transformation evidence used in manual assertion genetically encoded fluorescent electrophysiology assay evidence used in manual assertion genetically encoded fluorescent ion concentration sensor assay evidence used in manual assertion genetically encoded fluorescent sensor evidence used in manual assertion genomic context evidence used in manual assertion genomic systematic evolution of ligands by exponential amplification evidence used in manual assertion glutathione S-transferase pull-down assay evidence used in manual assertion green fluorescent protein fusion protein localization evidence used in manual assertion green fluorescent protein immunolocalization evidence used in manual assertion green fluorescent protein reporter gene assay evidence used in manual assertion heat capacity-based evidence used in manual assertion heterologous protein expression evidence used in manual assertion heterologous system uptake evidence used in manual assertion heteronuclear single quantum coherence spectroscopy evidence used in manual assertion Hi-C evidence used in manual assertion high throughput cell biology evidence used in manual assertion high throughput direct assay evidence used in manual assertion high throughput evidence used in manual assertion high throughput expression pattern evidence used in manual assertion high throughput genetic interaction phenotypic evidence used in manual assertion high throughput mass spectrometry evidence used in manual assertion high throughput mutant phenotypic evidence used in manual assertion high-performance liquid chromatography evidence used in manual assertion histochemistry evidence used in manual assertion histology evidence used in manual assertion host colonization assay evidence used in manual assertion host penetration assay evidence used in manual assertion host-surrogate penetration assay evidence used in manual assertion hydroxyl-radical footprinting evidence used in manual assertion Illumina sequencing evidence used in manual assertion imaging assay evidence used in manual assertion immuno-labelling electron microscopy evidence used in manual assertion immunocytochemistry evidence used in manual assertion immunodepletion evidence used in manual assertion immunofluorescence confocal microscopy evidence used in manual assertion immunofluorescence evidence used in manual assertion immunofluorescence super resolution microscopy evidence used in manual assertion immunofluorescence wide-field microscopy evidence used in manual assertion immunogold labelling electron microscopy assay evidence used in manual assertion immunogold labelling evidence used in manual assertion immunohistochemistry evidence used in manual assertion immunolocalization evidence used in manual assertion immunological assay evidence used in manual assertion immunoperoxidase immunolocalization electron microscopy evidence used in manual assertion immunoperoxidase immunolocalization evidence used in manual assertion immunoprecipitation evidence used in manual assertion imported information used in manual assertion in situ hybridization evidence used in manual assertion in vitro acetylation assay evidence used in manual assertion in vitro assay evidence used in manual assertion in vitro cleavage assay evidence used in manual assertion in vitro deacetylation assay evidence used in manual assertion in vitro defarnesylation assay evidence used in manual assertion in vitro demethylation assay evidence used in manual assertion in vitro desumoylation assay evidence used in manual assertion in vitro deubiquitination assay evidence used in manual assertion in vitro farnesylation assay evidence used in manual assertion in vitro methylation assay evidence used in manual assertion in vitro palmitoylation assay evidence used in manual assertion in vitro phosphatase assay evidence used in manual assertion in vitro polyADP-ribosylation assay evidence used in manual assertion in vitro protein kinase assay evidence used in manual assertion in vitro recombinant protein transcription reconstitution assay evidence used in manual assertion in vitro sumoylation assay evidence used in manual assertion in vitro transcription assay evidence used in manual assertion in vitro translation assay evidence used in manual assertion in vitro ubiquitination assay evidence used in manual assertion in vivo acetylation assay evidence used in manual assertion in vivo assay evidence used in manual assertion in vivo cleavage assay evidence used in manual assertion in vivo deacetylation assay evidence used in manual assertion in vivo defarnesylation assay evidence used in manual assertion in vivo demethylation assay evidence used in manual assertion in vivo desumoylation assay evidence used in manual assertion in vivo deubiquitination assay evidence used in manual assertion in vivo farnesylation assay evidence used in manual assertion in vivo methylation assay evidence used in manual assertion in vivo palmitoylation assay evidence used in manual assertion in vivo phosphatase assay evidence used in manual assertion in vivo polyADP-ribosylation assay evidence used in manual assertion in vivo protein kinase assay evidence used in manual assertion in vivo sumoylation assay evidence used in manual assertion in vivo transcription assay evidence used in manual assertion in vivo translation assay evidence used in manual assertion in vivo ubiquitination assay evidence used in manual assertion in-gel protein kinase assay evidence used in manual assertion indirect Coombs test evidence used in manual assertion infection assay evidence used in manual assertion inference based on individual clinical experience used in manual assertion inference by association of genotype from phenotype used in manual assertion inference from background scientific knowledge used in manual assertion inference of intermediate molecular structure from structure of final molecule used in manual assertion inference of MHC restriction based on shared MHC expression used in manual assertion inference of sequence features from visual inspection used in manual assertion inferential evidence used in manual assertion insertion mutation phenotypic evidence used in manual assertion interferometric reflectance imaging sensor evidence used in manual assertion intermethylated site amplification evidence used in manual assertion intrinsic disorder prediction evidence used in manual assertion intrinsic fluorescence-based protein conformation evidence used in manual assertion iodine-125-labeled ligand binding evidence used in manual assertion ion trap mass spectrometry evidence used in manual assertion isothermal titration calorimetry evidence used in manual assertion Kadouri drip-fed biofilm assay evidence used in manual assertion knockin evidence used in manual assertion knockout evidence used in manual assertion koehler illumination microscopy evidence used in manual assertion light scattering evidence used in manual assertion lipid binding assay evidence used in manual assertion liquid chromatography coupled with tandem mass spectrometry evidence used in manual assertion loss-of-function mutant phenotype evidence used in manual assertion luciferase reporter gene assay evidence used in manual assertion luminescence-based mammalian interactome mapping assay evidence used in manual assertion machine learning method evidence used in manual assertion machine learning prediction of motif instance evidence used in manual assertion macropatch voltage clamp recording evidence used in manual assertion macroscopic current trace evidence used in manual assertion macroscopy evidence used in manual assertion magnetic resonance evidence used in manual assertion mammalian 2-hybrid assay evidence used in manual assertion manually integrated combinatorial computational and experimental evidence used in manual assertion manually integrated combinatorial computational evidence used in manual assertion manually integrated combinatorial evidence used in manual assertion manually integrated combinatorial experimental evidence used in manual assertion mass spectrometry evidence used in manual assertion match to InterPro member signature evidence used in manual assertion match to sequence model evidence used in manual assertion matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in manual assertion medical imaging evidence used in manual assertion medical practitioner statement evidence used in manual assertion membrane fusion assay evidence used in manual assertion methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence used in manual assertion methylation interference footprinting evidence used in manual assertion methylation-specific polymerase chain reaction evidence used in manual assertion MHC complex binding prediction based MHC restriction evidence used in manual assertion MHC-specific blocking based MHC restriction evidence used in manual assertion MHC:ligand binding based MHC restriction evidence used in manual assertion microscale thermophoresis evidence used in manual assertion microscopy evidence used in manual assertion microtiter plate biofilm assay evidence used in manual assertion missense mutation pohenotypic evidence used in manual assertion mixed support of intron positions by RNA-sequencing alignment evidence used in manual assertion molecular dynamics evidence used in manual assertion morpholino experiment evidence used in manual assertion morphological similarity evidence used in manual assertion motif discovery evidence used in manual assertion motif similarity evidence used in manual assertion motility assay evidence used in manual assertion motility wound healing assay evidence used in manual assertion mRNA interactome capture evidence used in manual assertion MTS assay evidence used in manual assertion MTT assay evidence used in manual assertion multilocus sequence typing evidence used in manual assertion multiple sequence alignment evidence used in manual assertion multiplex bead-based immunoassay evidence used in manual assertion mutant phenotype evidence used in manual assertion mutant physiological response evidence used in manual assertion mutant visible phenotype evidence used in manual assertion nano-cap analysis of gene expression evidence used in manual assertion native protein gel electrophoresis evidence used in manual assertion natural variation mutant evidence used in manual assertion near-UV circular dichroism evidence used in manual assertion neural network method evidence used in manual assertion neutralization test assay evidence used in manual assertion Nimblegen array evidence used in manual assertion no evidence data found used in manual assertion nonsense mutation phenotypic evidence used in manual assertion northern blot evidence used in manual assertion Northwestern blot evidence used in manual assertion nuclear fragmentation evidence used in manual assertion nuclear magnetic resonance evidence used in manual assertion nuclear magnetic resonance imaging evidence used in manual assertion nuclear magnetic resonance spectroscopy evidence used in manual assertion nuclear magnetic resonance spectroscopy-based hydrogen-deuterium exchange evidence used in manual assertion nuclease protection assay evidence used in manual assertion nucleic acid binding evidence used in manual assertion nucleotide analog incorporation assay evidence used in manual assertion nucleotide BLAST evidence used in manual assertion optogenetic evidence used in manual assertion over expression analysis evidence used in manual assertion pairwise sequence alignment evidence used in manual assertion partial support of intron positions by RNA-sequencing alignment evidence used in manual assertion particle scattering evidence used in manual assertion particle size and count assay evidence used in manual assertion patch-clamp recording evidence used in manual assertion peptide affinity enrichment evidence used in manual assertion peptide array evidence used in manual assertion pH-induced protein unfolding evidence used in manual assertion phage display evidence used in manual assertion pharmacological assay evidence used in manual assertion phase contrast microscopy evidence used in manual assertion phenomic profiling assay evidence used in manual assertion phosphoamino acid analysis evidence used in manual assertion photoconversion evidence used in manual assertion phylogenetic determination of loss of key residues evidence used in manual assertion phylogenetic distribution evidence used in manual assertion phylogenetic footprinting evidence used in manual assertion physical examination evidence used in manual assertion physical interaction evidence used in manual assertion plaque assay evidence used in manual assertion plasmid maintenance assay evidence used in manual assertion point mutation phenotypic evidence used in manual assertion polyadenylated transcript 3'-end-sequencing evidence used in manual assertion polysome profiling evidence used in manual assertion position-specific scoring matrix motif search evidence used in manual assertion positional similarity evidence used in manual assertion primer extension assay evidence used in manual assertion propidium iodide staining evidence used in manual assertion protein binding evidence used in manual assertion protein BLAST evidence used in manual assertion protein dot blot assay evidence used in manual assertion protein expression evidence used in manual assertion protein expression-based MHC presentation evidence used in manual assertion protein fragment functional complementation evidence used in manual assertion protein hydrogen-deuterium exchange mass spectrometry evidence used in manual assertion protein inhibition evidence used in manual assertion protein mass spectrometry evidence used in manual assertion protein microarray evidence used in manual assertion protein separation followed by direct sequencing evidence used in manual assertion protein separation followed by fragment identification evidence used in manual assertion protein sequencing assay evidence used in manual assertion protein thermal shift assay evidence used in manual assertion protein unfolding evidence used in manual assertion protein-oligonucleotide microarray binding evidence used in manual assertion protein:ion binding evidence used in manual assertion proton-based nuclear magnetic resonance evidence used in manual assertion pulsed-field gel electrophoresis evidence used in manual assertion qualitative macroscopy evidence used in manual assertion qualitative western immunoblotting evidence used in manual assertion quantitative macroscopy evidence used in manual assertion quantitative mass spectrometry evidence used in manual assertion quantitative polymerase chain reaction evidence used in manual assertion quantitative reverse transcription polymerase chain reaction evidence used in manual assertion quantitative trait analysis evidence used in manual assertion quantitative western immunoblotting evidence used in manual assertion radioimmunoassay evidence used in manual assertion radioisotope assay evidence used in manual assertion radioisotope quantification assay evidence used in manual assertion radioligand binding assay evidence used in manual assertion Raman spectroscopy evidence used in manual assertion random mutagenesis evidence used in manual assertion random mutagenesis of specific target DNA evidence used in manual assertion rapid amplification of cDNA ends polymerase chain reaction evidence used in manual assertion rapid divergence from ancestral sequence evidence used in manual assertion recessive mutant phenotype evidence used in manual assertion reconstituted bilayer single-channel patch recording evidence used in manual assertion regular expression method evidence used in manual assertion regular expression motif search evidence used in manual assertion repeat expansion mutation phenotypic evidence used in manual assertion resonant mirror biosensor evidence used in manual assertion restriction fragment detection evidence used in manual assertion restriction landmark genomic scanning evidence used in manual assertion reverse transcription polymerase chain reaction evidence used in manual assertion ribohomopolymer binding assay evidence used in manual assertion ribosome profiling evidence used in manual assertion RNA detection assay evidence used in manual assertion RNA dot blot assay evidence used in manual assertion RNA protection assay evidence used in manual assertion RNA-protein binding evidence used in manual assertion RNA-sequencing evidence used in manual assertion RNAi evidence used in manual assertion rotary shadowing electron microscopy evidence used in manual assertion rule-based machine learning method evidence used in manual assertion S1 nuclease protection assay evidence used in manual assertion scanning electron microscopy evidence used in manual assertion selective antibody-based structural conformation evidence used in manual assertion self-reported ancestry evidence used in manual assertion self-reported individual's statement evidence used in manual assertion self-reported patient statement evidence used in manual assertion sequence alignment evidence used in manual assertion sequence orthology evidence used in manual assertion sequence similarity evidence used in manual assertion sexual sporulation assay evidence used in manual assertion silent mutation evidence used in manual assertion similarity evidence used in manual assertion single exon transcript confirmation via alignment evidence used in manual assertion single molecule fluorescence in situ hybridization evidence used in manual assertion single-cell RNA-sequencing evidence used in manual assertion single-unit extracellular recording evidence used in manual assertion site-directed mutagenesis phenotypic evidence used in manual assertion site-directed spin-labelling electron paramagnetic resonance evidence used in manual assertion slide agglutination test evidence used in manual assertion small interfering RNA knockdown evidence used in manual assertion small-angle neutron scattering evidence used in manual assertion small-angle X-ray scattering evidence used in manual assertion SOLiD sequencing evidence used in manual assertion southern hybridization evidence used in manual assertion Southwestern blot evidence used in manual assertion spatial pattern of protein expression evidence used in manual assertion spatial pattern of transcript expression evidence used in manual assertion specific MHC molecule based MHC restriction evidence used in manual assertion specific MHC protein expression-based MHC presentation evidence used in manual assertion specific protein inhibition by antibody evidence used in manual assertion spectrophotometry evidence used in manual assertion spheroplast fusion assay evidence used in manual assertion splice site mutation phenotypic evidence used in manual assertion split-ubiquitin functional complementation evidence used in manual assertion sporulation assay evidence used in manual assertion static fluorescence quenching evidence used in manual assertion static light scattering assay evidence used in manual assertion stopped-flow fluorescence spectroscopy evidence used in manual assertion string-matching method evidence used in manual assertion structural ensemble generation evidence used in manual assertion structural similarity evidence used in manual assertion subtractive hybridization evidence used in manual assertion super-resolution microscopy evidence used in manual assertion support of intron positions by RNA-sequencing alignment evidence used in manual assertion support vector machine evidence used in manual assertion suppressor/enhancer interaction phenotypic evidence used in manual assertion surface plasmon resonance evidence used in manual assertion survival rate analysis evidence used in manual assertion sustained current evidence used in manual assertion synchrotron radiation circular dichroism evidence used in manual assertion syngeneic transplantation experiment evidence used in manual assertion systematic evolution of ligands by exponential amplification evidence used in manual assertion T cell epitope assay type based MHC restriction evidence used in manual assertion T cell type based MHC restriction evidence used in manual assertion TACE activity assay evidence used in manual assertion targeting sequence prediction evidence used in manual assertion temperature jump relaxation evidence used in manual assertion temperature-induced protein unfolding evidence used in manual assertion temperature-sensitive mutant phenotypic evidence used in manual assertion terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence used in manual assertion tethered cell assay evidence used in manual assertion thin layer chromatography evidence used in manual assertion thioflavin T based fluorescence spectroscopy evidence used in manual assertion time-lapsed microscopy evidence used in manual assertion tissue ablation phenotypic evidence used in manual assertion tissue grafting phenotypic evidence used in manual assertion tissue microarray evidence used in manual assertion transcript expression evidence used in manual assertion transcriptional activation assay evidence used in manual assertion transgenic organism evidence used in manual assertion transgenic rescue experiment evidence used in manual assertion transient rescue experiment evidence used in manual assertion translocation mutation phenotypic evidence used in manual assertion transmembrane domain prediction evidence used in manual assertion transmission electron microscopy evidence used in manual assertion transmitted light brightfied mircoscopy evidence used in manual assertion tryptic phosphopeptide mapping assay evidence used in manual assertion tumble frequency assay evidence used in manual assertion turbidity measurement evidence used in manual assertion two-dimensional agarose gel electrophoresis evidence used in manual assertion two-dimensional polyacrylamide gel electrophoresis evidence used in manual assertion two-electrode voltage clamp recording evidence used in manual assertion ultraviolet light footprinting evidence used in manual assertion urea-induced protein unfolding evidence used in manual assertion urine test evidence used in manual assertion use dependence of inactivation evidence used in manual assertion viral hemagglutination inhibition assay evidence used in manual assertion viscosity measurement evidence used in manual assertion voltammetry evidence used in manual assertion voucher specimen phenotypic analysis evidence used in manual assertion whole transcript splice pattern evidence used in manual assertion whole-cell patch-clamp recording evidence used in manual assertion whole-cell voltage clamp recording evidence used in manual assertion wide-field fluorescence microscopy evidence used in manual assertion wide-field microscopy evidence used in manual assertion WST-1 assay evidence used in manual assertion x-ray crystallography evidence used in manual assertion X-ray crystallography-based structural model with high relative B-factor values used in manual assertion X-ray crystallography-based structural model with missing residue coordinates used in manual assertion xenotransplantation phenotypic evidence used in manual assertion xylE reporter gene assay evidence used in manual assertion yeast 2-hybrid evidence used in manual assertion yeast one-hybrid evidence used in manual assertion yellow fluorescent protein fusion protein localization evidence used in manual assertion zone of inhibition evidence used in manual assertion