Term Name: | 5-bromo-4-chloro-3-indolyl phosphate |
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Synonyms: | 5-Bcip, 5-bromo-4-chloro-1H-indol-3-yl dihydrogen phosphate, 5-Bromo-4-chloro-3-indoxyl phosphate, 5-bromo-4-chloroindoxyl phosphate, BCIP, X-Phos |
Definition: | An aryl phosphate that is indoxyl phopshate in which the indole moiety is substituted at positions 4 and 5 by chlorine and bromine, respectively. It is used to test for the presence of an enzyme, alkaline phosphatase, which cleaves the phosphate group to give 5-bromo-4-chloroindoxyl, which immediately dimerises to give an intensely blue product. |
Ontology: | ChEBI [CHEBI:75508] ( EBI ) |