UniProt ID: B2GQK5 |
CATALYTIC ACTIVITY: Reaction=3-O-(alpha-L-fucosyl)-L-seryl-[EGF-like domain protein] + UDP- N-acetyl-alpha-D-glucosamine = 3-O-(N-acetyl-beta-D-glucosaminyl- (1->3)-alpha-L-fucosyl)-L-seryl-[EGF-like domain protein] + H(+) + UDP; Xref=Rhea:RHEA:70511, Rhea:RHEA-COMP:17919, Rhea:RHEA- COMP:17920, ChEBI:CHEBI:15378, ChEBI:CHEBI:57705, ChEBI:CHEBI:58223, ChEBI:CHEBI:189632, ChEBI:CHEBI:189633; EC=2.4.1.222; Evidence={ECO:0000256|ARBA:ARBA00033626, ECO:0000256|PIRNR:PIRNR038073}; CATALYTIC ACTIVITY: Reaction=3-O-(alpha-L-fucosyl)-L-threonyl-[EGF-like domain protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-(N-acetyl-beta-D-glucosaminyl- (1->3)-alpha-L-fucosyl)-L-threonyl-[EGF-like domain protein] + H(+) + UDP; Xref=Rhea:RHEA:70531, Rhea:RHEA-COMP:17922, Rhea:RHEA- COMP:17923, ChEBI:CHEBI:15378, ChEBI:CHEBI:57705, ChEBI:CHEBI:58223, ChEBI:CHEBI:189631, ChEBI:CHEBI:189634; EC=2.4.1.222; Evidence={ECO:0000256|ARBA:ARBA00033663, ECO:0000256|PIRNR:PIRNR038073}; COFACTOR: Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000256|ARBA:ARBA00001936, ECO:0000256|PIRNR:PIRNR038073, ECO:0000256|PIRSR:PIRSR038073-3}; SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000256|PIRNR:PIRNR038073}. Membrane {ECO:0000256|ARBA:ARBA00004606}; Single-pass type II membrane protein {ECO:0000256|ARBA:ARBA00004606}. SIMILARITY: Belongs to the glycosyltransferase 31 family. {ECO:0000256|PIRNR:PIRNR038073}. |
UniProt ID: Q8JHF2 |
FUNCTION: Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. Involved in the correct formation of boundaries in the somites and hindbrain (By similarity). Required for Delta-Notch-mediated induction of hypochord cells at the lateral borders of the midline precursor domain (PubMed:11429294, PubMed:14518000). {ECO:0000250|UniProtKB:O09010, ECO:0000269|PubMed:11429294, ECO:0000269|PubMed:14518000}. CATALYTIC ACTIVITY: Reaction=3-O-(alpha-L-fucosyl)-L-threonyl-[EGF-like domain protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-(N-acetyl-beta-D-glucosaminyl- (1->3)-alpha-L-fucosyl)-L-threonyl-[EGF-like domain protein] + H(+) + UDP; Xref=Rhea:RHEA:70531, Rhea:RHEA-COMP:17922, Rhea:RHEA- COMP:17923, ChEBI:CHEBI:15378, ChEBI:CHEBI:57705, ChEBI:CHEBI:58223, ChEBI:CHEBI:189631, ChEBI:CHEBI:189634; EC=2.4.1.222; CATALYTIC ACTIVITY: Reaction=3-O-(alpha-L-fucosyl)-L-seryl-[EGF-like domain protein] + UDP- N-acetyl-alpha-D-glucosamine = 3-O-(N-acetyl-beta-D-glucosaminyl- (1->3)-alpha-L-fucosyl)-L-seryl-[EGF-like domain protein] + H(+) + UDP; Xref=Rhea:RHEA:70511, Rhea:RHEA-COMP:17919, Rhea:RHEA- COMP:17920, ChEBI:CHEBI:15378, ChEBI:CHEBI:57705, ChEBI:CHEBI:58223, ChEBI:CHEBI:189632, ChEBI:CHEBI:189633; EC=2.4.1.222; COFACTOR: Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250}; SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250}; Single- pass type II membrane protein {ECO:0000250}. TISSUE SPECIFICITY: In the embryo, expressed along the A-P axis of the neural tube, within the lateral plate mesoderm, in the presomitic mesoderm and the somites, in specific rhombomeres of the hindbrain (even-numbered rhombomeres) and in the otic vesicles. {ECO:0000269|PubMed:11429294, ECO:0000269|PubMed:15376327}. DEVELOPMENTAL STAGE: Expressed both maternally and throughout the zygotic stages, with highest expression at the bud stage. No evidence for a cyclic pattern of expression in the presomitic mesoderm during somitogenesis. Expressed weakly at the epiboly stage (4.5 hours) throughout the blastoderm. Expression clears from marginal region and localizes to the epiblast cells of the animal pole. As gastrulation proceeds, expressed in hypoblast cells that migrate towards the animal pole, in the prechordal plate and in forerunner cells. At 90% epiboly, expressed in the neural plate. At the three somite stage (11 hours), expressed in a small cluster of cells within the tailbud region and in future spinal cord. From the 8-14 somite stages, expressed in alternating pre-rhombomeres of the hindbrain, and more broadly in mid- and forebrain. Also expressed weakly in the anterior margins of the formed somites and in the anterior presomitic mesoderm. At the end of somitogenesis (22 hours), expressed strongly in the telencephalon and anterior midbrain, weakly in other parts of the midbrain and variably in the hindbrain. {ECO:0000269|PubMed:11429294, ECO:0000269|PubMed:11702199, ECO:0000269|PubMed:14518000, ECO:0000269|PubMed:16729214}. PTM: A soluble form may be derived from the membrane form by proteolytic processing. {ECO:0000305}. SIMILARITY: Belongs to the glycosyltransferase 31 family. {ECO:0000305}. |
This information was provided by UniProt through a collaboration with ZFIN. (1) |