UniProt ID: F1RET2 |
FUNCTION: Histone methyltransferase that specifically trimethylates 'Lys-20' of histone H4 to form trimethylated histone H4 lysine 20 (H4K20me3) which represents a specific tag for epigenetic transcriptional repression (By similarity). Plays a crucial role in hematopoiesis during embryogenesis by negatively regulating expression of genes related to both primitive and definitive hematopoiesis (PubMed:27377701). {ECO:0000250|UniProtKB:Q3TYX3, ECO:0000269|PubMed:27377701}. CATALYTIC ACTIVITY: Reaction=L-lysyl(20)-[histone H4] + 3 S-adenosyl-L-methionine = 3 H(+) + N(6),N(6),N(6)-trimethyl-L-lysyl(20)-[histone H4] + 3 S-adenosyl-L- homocysteine; Xref=Rhea:RHEA:64456, Rhea:RHEA-COMP:15554, Rhea:RHEA- COMP:15998, ChEBI:CHEBI:15378, ChEBI:CHEBI:29969, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:61961; EC=2.1.1.372; Evidence={ECO:0000250|UniProtKB:Q3TYX3}; TISSUE SPECIFICITY: Expressed at high levels in the ovary and at lower levels in the fin, testis and brain. {ECO:0000269|PubMed:27377701}. DEVELOPMENTAL STAGE: Abundantly expressed at early developmental stages but decreases slightly when embryos proceed in development (PubMed:27377701). Expression is strongly detected from 0.25 to 3 hours post-fertilization (hpf) in embryos, but it is only weakly observed at 12 hpf (PubMed:27377701). At 24 and 36 hpf, signals are observed only around the eye with stronger intensities at 24 hpf than 36 hpf (PubMed:27377701). {ECO:0000269|PubMed:27377701}. DISRUPTION PHENOTYPE: Morpholino knockdown results in embryos which show normal gross morphological development, including of heart and skeletal muscle (PubMed:27377701). However, an increased expression of genes related to both primitive and definitive hematopoiesis is seen (PubMed:27377701). {ECO:0000269|PubMed:27377701}. SIMILARITY: Belongs to the class V-like SAM-binding methyltransferase superfamily. {ECO:0000255|PROSITE-ProRule:PRU00190}. |
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