FIGURE SUMMARY
Title

Ontogeny and behaviour of early macrophages in the zebrafish embryo

Authors
Herbomel, P., Thisse, B., and Thisse, C.
Source
Full text @ Development

Expression patterns of the draculin and L-plastin genes in the zebrafish embryo. (A-K) Expression of the draculin gene. (A-C) Gastrulation and early segmentation period, lateral view, animal pole on top, dorsal side to the right. (A) 75% epiboly; draculin is expressed in a large domain of ventral and ventro-lateral hypoblast, and in the borders of the prechordal plate (p). (B) 90% epiboly; this embryo was slightly tilted (a cross marks the animal pole) to make the prechordal plate (p) visible; the draculin expression domain displays a tip towards the animal pole that will become anterior lateral mesoderm. (C) 3-somite stage; dra is no longer expressed in the prechordal plate; two dra domains become apparent in lateral mesoderm: a larger one posteriorly, and a smaller one anteriorly. (D-K) Segmentation period, dorsal view, anterior to the top. (D-F) Posterior dra expression domain at 3-somite (D), 8-somite (E) and 13-somite (F) stages. (G-K) Anterior dra expression domain at 3-somite (G), 8-somite (H), 11-somite (I), 15-somite (J) and 20-somite (K) stages. (M,N) Anterior flk expression at 11-somite (M) and 15-somite (N) stages; the bottom spot centered on the midline is not in lateral mesoderm but in the brain. (O,P) Anterior expression of dra+flk at 11-somite (O) and 15-somite (P) stages; ao, aortic arch primordia. (L,Q-T) Expression of the L-plastin gene. (L) Dorsal view, anterior to the top, at 20-somite stage. (Q-T) Lateral view at 24 hpf (30-somite stage, Q,R), 28 hpf (S) and 32 hpf (T); the focus is on the yolksac in Q and on the embryo in R,T. In S, differently focused pictures were fused electronically to show L-plastin positive cells in the embryo and yolksac simultaneously; the arrow shows the newly appeared caudal group of L-plastin positive cells.

Acknowledgments
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