Raz et al., 1998 - ß-lactamase as a marker for gene expression in live zebrafish embryos. Developmental Biology   203:290-294 Full text @ Dev. Biol.

Fig. 1 Rapid detection of β-lactamase gene expression. (A–D) One-cell-stage embryos were injected with 1 mM CCF2 (right embryo) or with 1 mM CCF2 + 10 ng/μl pXex–β-lactamase plasmid DNA (left embryo). Detection with the CCF2 filter set at the oblong stage (3.7 h at 28.5°C) (A), sphere (4 at 28.5°C) (B), 30% epiboly (4 h at 28.5°C) (C), 80% epiboly (8.5 h at 28.5°C) (D), and 10 somites stage (14 h at 28.5°C) (E). (F–H) One cell stage embryos were injected with 10 ng/μl of pXex–mmGFP plasmid and fluorescence was detected using the fluorescein filter set at 50% epiboly (5.3 h at 28.5°C) (F), 80% epiboly (8.5 h at 28.5°C) (G) and at the 10 somites stage (14 h at 28.5°C) (H).

Fig. 2 Cell-autonomous detection of the β-lactamase marker. (A) One-cell-stage embryos were injected with CCF2 and later at the 16-cell stage one of the marginal blastomeres was injected with β-lactamase mRNA. At this stage the cytoplasm of the marginal blastomeres is not separated allowing the mRNA to diffuse and to be translated in more than 1/16 of the cells. (B, C) One-cell-stage embryos were injected with CCF2 and later at the 64-cell stage one of the blastomeres was coinjected with β-lactamase mRNA and with rhodamine dextran as a lineage marker. At 30% epiboly the yolk was removed and a flat preparation of the embryo was visualized at a high magnification using the CCF2 filter set (B) or with a filter set for rhodamine detection (C).

Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 203, Raz, E., Zlokarnik, G., Tsien, R.Y., and Driever, W., ß-lactamase as a marker for gene expression in live zebrafish embryos, 290-294, Copyright (1998) with permission from Elsevier. Full text @ Dev. Biol.