- Title
-
GATA-1 expression pattern can be recapitulated in living transgenic zebrafish using GFP reporter gene
- Authors
- Long, Q.M., Meng, A.M., Wang, H., Jessen, J.R., Farrell, M.J., and Lin, S.
- Source
- Full text @ Development
Zebrafish GATA-1 promoter activity in living zebrafish embryos. (A) Transient GFP expression (fluorescent green) driven by a zebrafish GATA-1 promoter construct (G1-GM2) in an 8-hour-old embryo. (B,C) Transient GFP expression in the intermediate cell mass of zebrafish embryos (construct G1-GM2). (D) Transient GFP expression in circulating blood cells of a 2-day-old zebrafish embryo. A white arrow indicates circulation in the dorsal aorta and red arrows indicate circulation in the the ventral axial vein. |
Comparison of GATA-1 mRNA and GFP expression driven by the GATA-1 promoter in germline transgenic zebrafish. (A,B) GFP expression driven by the G1-GM2 construct in a 12 hour and a 22 hour transgenic zebrafish embryo, respectively. (C,D) GATA-1 mRNA detected by RNA in situ hybridization in a 12 hour and a 24 hour zebrafish embryo, respectively. (E) GFP expression in circulating blood cells in the ventral axial vein of a 3-day-old transgenic embryo. (F) GFP expression in circulating blood cells of an adult transgenic zebrafish. EXPRESSION / LABELING:
|
GFP expression in blood cells of transgenic zebrafish harboring the G1-GM2 construct. (A,B) GFP expression in blood cells isolated from 48 hour embryos and adult transgenic fish, respectively. (C) GFPnegative blood cells from a non-transgenic fish (D) GFP expression in a cross section of head kidney (arrows) from a 1-month-old transgenic zebrafish. (E) Cross section of liver from the same transgenic fish. EXPRESSION / LABELING:
|