Isolation-Raised Larvae Have a Larger Social Avoidance Area (A) Groups of seven larvae raised either in groups or in isolation were recorded for 1 h while swimming in a circular arena 50 mm in diameter. (B) The difference in density distributions of positions of all other larvae relative to a focal larva was calculated between real and temporally shuffled data for group-raised (top row) and isolation-raised larvae (bottom row). The center panel shows a zoomed-in section of the density distribution. The dot in the center indicates the position of the focal fish facing upward. The avoidance area was quantified as the area where the data point density distribution is below 60% of the shuffled data. Shown on the left are the overlaid contours of the avoidance area calculated for each group of seven larvae in the two raising conditions. (C) Boxplots depict the quartiles of avoidance area measures comparing the effect of the raising condition. Whiskers signal the range of data, and + indicates the mean per condition. Asterisks signal the results of a two-tailed unsigned Wilcoxon test between raising conditions (alpha level 0.001), sample size: n = 9, N = 63. See also Figure S1 and Table S1.