FIGURE SUMMARY
Title

Modeling ZNF408-Associated FEVR in Zebrafish Results in Abnormal Retinal Vasculature

Authors
Karjosukarso, D.W., Ali, Z., Peters, T.A., Zhang, J.Q.C., Hoogendoorn, A.D.M., Garanto, A., van Wijk, E., Jensen, L.D., Collin, R.W.J.
Source
Full text @ Invest. Ophthalmol. Vis. Sci.

Photoreceptor characterization in adult wild-type and znf408-mutant zebrafish. (a) A representative image of Bodipy staining per condition is shown. Scale bar is 25 µm. (b) Quantification of OS length. At least 3 sections were analyzed per condition. *P < 0.05. (c) A representative image of Gnat2 staining per condition is shown. Scale bar is 25 µm.

Progressive vascular pathology in znf408-mutant zebrafish larvae and adults. (a) Confocal micrographs at 5, 90, or 180 days postfertilization (dpf) of the hyaloid (5 dpf, top row) or retinal vessels (90 dpf, second row and 180 dpf, third row) in znf408+/+znf408rmc105/+znf408rmc105/rmc105znf408rmc103/rmc103, and znf408rmc104/rmc104 zebrafish crossed onto the Tg(flk1:GFP) background (vessels shown in green) following injection with rhodamine-conjugated dextran (plasma shown in red). (b) Quantification of the number of hyaloid vessels at 5 dpf from the groups shown in (a). n = 10, ANOVA: P < 0.001, Dunnett's post hoc: ***P < 0.001. (c) Quantification of the number of sprouts in the retinal vessels at 90 dpf from the groups shown in (a). n = 10, ANOVA: P < 0.001, Dunnett's post hoc: ***P < 0.001. (d) Quantification of the leaked dextran as a percentage of total dextran in the retinal vasculature at 180 dpf from the groups shown in (a). n = 10, ANOVA: P < 0.001, Dunnett's post hoc: ***P < 0.001.

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Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Invest. Ophthalmol. Vis. Sci.