Karjosukarso et al., 2020 - Modeling ZNF408-Associated FEVR in Zebrafish Results in Abnormal Retinal Vasculature. Investigative ophthalmology & visual science   61:39 Full text @ Invest. Ophthalmol. Vis. Sci.

Fig. 3

Photoreceptor characterization in adult wild-type and znf408-mutant zebrafish. (a) A representative image of Bodipy staining per condition is shown. Scale bar is 25 µm. (b) Quantification of OS length. At least 3 sections were analyzed per condition. *P < 0.05. (c) A representative image of Gnat2 staining per condition is shown. Scale bar is 25 µm.

Fig. 4

Progressive vascular pathology in znf408-mutant zebrafish larvae and adults. (a) Confocal micrographs at 5, 90, or 180 days postfertilization (dpf) of the hyaloid (5 dpf, top row) or retinal vessels (90 dpf, second row and 180 dpf, third row) in znf408+/+znf408rmc105/+znf408rmc105/rmc105znf408rmc103/rmc103, and znf408rmc104/rmc104 zebrafish crossed onto the Tg(flk1:GFP) background (vessels shown in green) following injection with rhodamine-conjugated dextran (plasma shown in red). (b) Quantification of the number of hyaloid vessels at 5 dpf from the groups shown in (a). n = 10, ANOVA: P < 0.001, Dunnett's post hoc: ***P < 0.001. (c) Quantification of the number of sprouts in the retinal vessels at 90 dpf from the groups shown in (a). n = 10, ANOVA: P < 0.001, Dunnett's post hoc: ***P < 0.001. (d) Quantification of the leaked dextran as a percentage of total dextran in the retinal vasculature at 180 dpf from the groups shown in (a). n = 10, ANOVA: P < 0.001, Dunnett's post hoc: ***P < 0.001.

Fig. 5 ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Acknowledgments:
ZFIN wishes to thank the journal Investigative ophthalmology & visual science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Invest. Ophthalmol. Vis. Sci.