FIGURE SUMMARY
Title

A robust human norovirus replication model in zebrafish larvae

Authors
Van Dycke, J., Ny, A., Conceição-Neto, N., Maes, J., Hosmillo, M., Cuvry, A., Goodfellow, I., Nogueira, T.C., Verbeken, E., Matthijnssens, J., de Witte, P., Neyts, J., Rocha-Pereira, J.
Source
Full text @ PLoS Pathog.
Fig 5

HuNoV sites of replication.

(A) Ten HuNoV-injected larvae were deyolked and then dissected into head, body, and tail (as depicted in the scheme) at 3 days pi. Bars represent the viral RNA levels/zebrafish larva, quantified by RT-qPCR (7 independent experiments). (B) Anatomy of a 6 dpf zebrafish larva.
Fig 6

HuNoV detected in the liver and pancreas of infected zebrafish larvae at 3 days pi.

Immunohistochemistry of HuNoV GII.P7-GII.6-infected zebrafish larvae (A, C, E) harvested at day 3 pi [and the respective uninfected controls (B, D, F)] was performed. Images show 5 μm sections stained with antibodies targeting VP1 at 10x and 40x magnifications, insets at 100x magnification of sagittal sections (A-D) and coronal sections (E-F). Viral antigens were detected in the liver (A, C, D, E) and pancreas (A, C). L: liver, P: pancreas, SB: swim bladder, I: intestine.
Fig 7

HuNoV detected in the intestine and caudal hematopoietic tissue of infected zebrafish larvae at 3 days pi.

Immunohistochemistry of HuNoV GII.P7-GII.6-infected zebrafish larvae (A, C, E, G, I) harvested at day 3 pi [and the respective uninfected controls (B, D, F, H, J)] was performed. Images show 5 μm sections stained with antibodies targeting VP1 at 10x and 40x magnifications, insets at 100x magnification of sagittal sections (A-F) and coronal sections (G-J). Viral antigens were detected in the intestine (A, C, G) and caudal hematopoietic tissue (A, E, I). L: liver, C: cloaca, SB: swim bladder, I: intestine, Pi: pigment, CHT: caudal hematopoietic tissue.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Pathog.
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