FIGURE SUMMARY
Title

Gene expression profiling reveals a conserved microglia signature in larval zebrafish

Authors
Mazzolini, J., Le Clerc, S., Morisse, G., Coulonges, C., Kuil, L.E., van Ham, T.J., Zagury, J.F., Sieger, D.
Source
Full text @ Glia

Development of the microglia population in larval zebrafish. (a) Representative confocal images are shown to illustrate zebrafish larval head development from 1 to 7 days postfertilization (dpf) and microglial cell distribution throughout the developing brain. Upper panels correspond to a brightfield transmission image and lower panels represent the maximum intensity projection of 4C4+ microglia (magenta) at each developmental stage. Microglia start colonizing the brain (dotted line) at 3 dpf whereas signal can be detected in the retina from 1 dpf onwards. Scale bar represents 50 μm. (b) Upper panels show 4C4 antibody immunohistochemistry, lower panels show segmented images of microglia morphology at 3, 5, and 7 dpf using the Imaris surface tool. Microglia morphology changes from amoeboid (1 dpf) to ramified (7 dpf) with an intermediate feature at 5 dpf. Scale bar represents 10 μm. (c) Schematic representation of the protocol used to isolate 4C4+ microglia from larval zebrafish brains at 3, 5, and 7 dpf. All images represent maximum intensity projections of confocal stacks. Images were captured using a Zeiss LSM710 confocal microscope with a 20×/NA 0.8 objective [Color figure can be viewed at http://wileyonlinelibrary.com]

EXPRESSION / LABELING:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Days 7-13

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EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Days 7-13
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Glia