The prostaglandin E2 dependent growth defect of Δplb1 is also present in vivo.

A i H99-GFP infected larvae imaged at 0, 1, 2 and 3 dpi. At least 50 larvae measured per time point across 3 biological repeats. Box and whiskers show median, 5th percentile and 95th percentile. Unpaired Mann-Whitney U tests used to compare the burden between each strain for every time point, for p values see (S1Bii Fig). B i– H99-GFP Infected larvae treated with 10 μM prostaglandin E2 or equivalent solvent (DMSO) control. At least 60 larvae measured per treatment group from 3 biological repeats. Box and whiskers show median, 5th percentile and 95th percentile. Unpaired Mann-Whitney U tests used to compare between treatments DMSO vs. 10 μM PGE2 * p = 0.0137 (threshold for significance 0.017, corrected for multiple comparisons). C i, H99-GFP Infected larvae treated with 10 μM prostaglandin D2 or equivalent solvent (DMSO) control. At least 60 larvae measured per treatment group from 4 biological repeats. Box and whiskers show median, 5th percentile and 95th percentile. Unpaired Mann-Whitney U tests used to compare between treatments DMSO vs. 10 μM PGD2., ns p = 0.8 D iΔplb1-GFP infected larvae (500 cell inoculum injected at 2 dpf) imaged at 0, 1, 2 and 3 dpi. N = 3. Box and whiskers show median, 5th percentile and 95th percentile. At least 87 larvae measured for each time point from 3 biological repeats. Unpaired Mann-Whitney U tests used to compare the burden between each strain for every time point, for p values see (S1Ai and S1Aii Fig). E i Δplb1-GFP Infected larvae treated with 10 μM prostaglandin E2 or equivalent solvent (DMSO) control. At least 35 larvae measured per treatment group from 2 biological repeats. Box and whiskers show median, 5th percentile and 95th percentile. Unpaired Mann-Whitney U tests used to compare between treatments Δplb1-GFP DMSO vs 10 μM PGE2 *** p = 0.0001 (threshold for significance 0.017, corrected for multiple comparisons). F i Δplb1-GFP Infected larvae treated with 10 μM prostaglandin D2 or equivalent solvent (DMSO) control. At least 45 larvae measured per treatment group from 3 biological repeats. Box and whiskers show median, 5th percentile and 95th percentile. Unpaired Mann-Whitney U tests used to compare between treatments DMSO vs. 10 μM PGD2. Ns p = 0.1 A ii Representative GFP images (representative = median value) H99-GFP infected larvae, untreated at 0,1,2,3 dpi. B ii, C ii Representative GFP images (representative = median value) H99-GFP infected larvae, at 2 dpi treated with 10 μM PGE2 (B ii) or PGD2 (C ii). D iiRepresentative GFP images (representative = median value) Δplb1-GFP infected larvae, untreated at 0,1,2,3 dpi. E ii, F ii Representative GFP images (representative = median value) Δplb1-GFP infected larvae, at 2 dpi treated with 10 μM PGE2 (E ii) or PGD2 (F ii).