Loss of MCU Limits mCa2+ Uptake and Elevates Total Body Fat in Zebrafish
(A) Schematic of the generation of global MCU KO zebrafish using CRISPR/Cas9.
(B) Genotyping for MCU deletion.
(C) Bar graph showing MCU mRNA abundance in WT, MCU+/−, and MCU−/− zebrafish.
(D) Western blot for MCU expression. n = 3.
(E) Measurement of mCa2+ uptake. n = 5–10.
(F) Quantification of ionomycin-induced peak mCa2+ levels from (E). n = 5–10.
(G) Bar graph representing cellular ATP levels in cells isolated from MCU+/+, MCU+/−, and MCU−/− zebrafish. n = 4.
(H) Adult WT and MCU−/− zebrafish were homogenized and centrifuged. MCU−/− zebrafish samples show a clear yellow color lipid layer on top of the protein lysate. n = 6.
(I) Adult WT and MCU−/− zebrafish were stained with Lipid Green to monitor the distribution of lipids in the whole body. n = 3.
(J and K) Bar graphs represent the quantification of Lipid Green staining from the dorsal (J) or tail (K) fin. n = 3.
Statistical analysis: mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
|Acknowledgments:||ZFIN wishes to thank the journal Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Rep.|