Mohd Fuad et al., 2018 - Characterization of 3D-Printed Moulds for Soft Lithography of Millifluidic Devices. Micromachines   9(3) Full text @ Micromachines (Basel)

Fig. 3

Fabrication of miniaturized 3D cages that mirror the shape of the biological specimens such as the larval stages of zebrafish (Danio rerio) using SLA moulds: (A) Microphotograph of a positive relief pattern fabricated using a ProJet 7000 HD in VisiJet SL Clear resin. The mould is the size of a standard microscope slide 25 mm × 75 mm. The dashed box denotes a single 3D cage; (B) Topographic surface analysis of a single 3D cage (marked as dashed box in (A) using scanning electron microscopy (SEM); (C) D. rerio larvae immobilized inside a PDMS cage, made from an SLA mould and that mirrors the organisms shape; and (D) High-resolution fluorescence imaging of a transgenic Fli1a:EGFP D. rerio larva showing green fluorescent protein (GFP)-expressing vasculature. Larger and smaller vessels are clearly distinguishable in the trunk region (boxed area, red outline).

ZFIN wishes to thank the journal Micromachines for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Micromachines (Basel)