O'Connor et al., 2018 - Bypassing Glutamic Acid Decarboxylase 1 (Gad1) Induced Craniofacial Defects with a Photoactivatable Translation Blocker Morpholino. ACS Chemical Neuroscience   10(1):266-278 Full text @ ACS Chem. Neurosci.

Fig. 5

Knockdown of Gad1 causes defects in head development and outgrowth visible at 1 dpf (A) wild-type; (B) gad1b splice blocking MO; (C) gad1b translation blocking MO; (D,E) BHQ-gad1b-ccMO; (F) gad1b mismatch control for splice blocking MO; (G,H) CyHQ-gad1b-ccMO; (I) gad1b mismatch control for translation blocking MO. (D,G) BHQ- and CyHQ-gad1b-ccMO injected embryos raised in the dark exhibit morphological development comparable to wild-type embryos. (E, H) BHQ- and CyHQ-gad1b-ccMO injected embryos raised in the light exhibit morphological development comparable to embryos injected with gad1b splice blocking or translation blocking MOs (B–C). Asterisk denotes regions exhibiting altered distribution of the presumptive head mesenchyme; bracket denotes altered gross structure of the midbrain-hindbrain region. Normal development is observed in embryos injected with 5 base mismatch controls for the gad1b splice blocking MO (F) or translation blocking MO (I). Scale bar in (A) applies to all panels. t, telencephalon; e, eye; m, midbrain; h, hindbrain; o, otocyst.

Fig. 6

Knockdown of Gad1 causes jaw defects visible at 3 dpf (A) wild-type; (B) gad1b morphant; (C,D) BHQ-gad1b-ccMO; (E,F) CyHQ-gad1b-ccMO. (C,E) BHQ- and CyHQ-gad1b-ccMO injected embryos raised in the dark exhibit morphological development comparable to wild-type embryos. (B, D, F). BHQ- and CyHQ-gad1b-ccMO injected embryos raised in the light exhibit abnormal development of the presumptive lower jaw (region denoted by bracket) comparable to embryos injected with gad1b MOs; region includes Meckel’s cartilage. Scale bar in (A) applies to all panels.

PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. 7

Morphological development of craniofacial features in the developing zebrafish probed by CyHQ-gad1b-ccMO. (A,B) Control fish. (B) Alcian blue stained, flat mount preparation showing normal development of Meckel’s (m), basihyal (bh), and palatoquadrate (pq) cartilages. (C,D) The gad1b morphant fish exhibit reduced Meckel’s and basihyal cartilages (denoted with brackets) with midlines defects. Note irregular organization of the cells in the Meckel’s cartilage of gad1b morphant fish (asterisk in D and H) compared to controls or ccMO injected fish photoactivated at 1 dpf.

 

Fig. 8 ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Fig. S22 ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: 10-13 somites
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: 10-13 somites

Fig. S26 ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Acknowledgments:
ZFIN wishes to thank the journal ACS Chemical Neuroscience for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ ACS Chem. Neurosci.