Sun et al., 2018 - An Assay for Systematically Quantifying the Vestibulo-Ocular Reflex to Assess Vestibular Function in Zebrafish Larvae. Frontiers in Cellular Neuroscience   12:257 Full text @ Front. Cell. Neurosci.

Fig. 2

(A) The head and eyes (red arrows) of a zebrafish larva acquired in a video series by the VOR test system. (B) The ROI containing the eyes is selected and used to analyze the eye movements and VOR. (C) The binary image of image in B (black eyes). (D) The inversed binary image of image in C (white eyes). (E) The inversed binary image in D, where the small flecks are removed by applying the area threshold, and the image is subsequently used to analyze the movements of the eyes stimulated by the rotation. Scale bars: in (A) 500 μm for A; in B, 200 μm for (B–E).

PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Day 6

Fig. 6

Lateral views, anterior toward the left, of OVs in 6dpf WT, dlx3b morphant and dlx4b morphant are shown. Most dlx3b morphants lacks their utricular otolith, while dlx4b morphants retain both otoliths. Scale bars: in A, 100 μm for A–C.

PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Day 6
Acknowledgments:
ZFIN wishes to thank the journal Frontiers in Cellular Neuroscience for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Front. Cell. Neurosci.