Bakuchiol inhibited in vivo cell metastasis in zebrafish. (A) Representative images of zebrafish embryo (a) without and (b) with cell metastasis. (B) Approximately 500 unsorted MCF-7 cells were injected into the yolk sacs of 48 hpf zebrafish embryos. Then, 40 embryos were treated with ethanol (solvent control) or with 0.5 or 1 μg/mL of bakuchiol for 7 days. Each condition was performed in triplicate. Statistical analysis of (a) the percentages of embryos without cell metastasis in control and treatment groups, and of (b) the numbers of fluorescent particles in control and treatment groups (^∗∗∗p < 0.001, ^∗∗p < 0.01, ^∗p < 0.05, one-way ANOVA). BAK, bakuchiol.