NOD1 is critical for embryo hatching and larvae survival. (a) The images of embryos and larvae from WT and NOD1-1IS ^−/− zebrafish at 3 days post-fertilisation (dpf). (b) The hatching rate of WT and NOD1-1IS ^−/− zebrafish at 2, 3 and 4 dpf (n = 4, each with 50 embryos). Data represent the mean ± the SEM, and were tested for statistical significance using two-tailed Student’s t-test. **p < 0.01. (c) The embryo survival curves of WT and NOD1-1IS ^−/− zebrafish (n = 4, each with 50 embryos). (d) The larval survival curves of WT and NOD1-1IS ^−/− zebrafish (n = 4, each with 49~52 larvae). The survival curves were compared statistically significant difference using the Log-Rank Test.