In vivo action of the Ptgs inhibitors on hCG induced oocyte maturation and ovulation. a, The levels of PGE2 after injection of Rp-cAMP (0.1 mM) in ovary. b, The levels of PGE2 after injection of SC-560 (2 mg/ml), NS-398 (2 mg/ml) and IM (2 mg/ml) in ovary. c, Oocyte ovulation rates in zebrafish injection with hCG(10IU/ml) or inhibitor alone for 3 h. At least 50 FG stage follicles, M stage follicles or oocytes from each replicate were scored for percentage ovulation. d. Oocyte ovulation rates in zebrafish injection with hCG(10IU/ml) +DMSO or hCG + inhibitor for 3 h. At least 50 FG stage follicles, M stage follicles or oocytes from each replicate were scored for percentage ovulation. e. Microscopic examination of follicles or ovary from zebrafish injection with hCG(10IU/ml) +DMSO or hCG + inhibitor and ovary histology from zebrafish injection with hCG + DMSO or hCG + inhibitor. The mature oocytes indicated by red arrow while ovulated oocytes indicated by black arrow. SC-560: a selective Ptgs1 inhibitor; NS-398: a selective Ptgs2 inhibitor; IM: Indomethacin, non-selective Ptgs inhibitor. Each value represents the mean value ± SEM of triplicate assays from 3 independent experiments. (***, P < 0.001 vs control). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)