FIGURE SUMMARY
Title

Internal epitope tagging informed by relative lack of sequence conservation

Authors
Burg, L., Zhang, K., Bonawitz, T., Grajevskaja, V., Bellipanni, G., Waring, R., Balciunas, D.
Source
Full text @ Sci. Rep.
Fig. 4

Validation of internally epitope tagged Tcf21 and Tb18.

(a–d) Internally epitope tagged Tcf21 is fully functional and complements corresponding frameshift mutations. Lateral (a,b) and ventral (c,d) views of 5 dpf zebrafish embryos, with cartilage stained with Alcian Blue. Embryos trans-heterozygous for V5-tcf21 and tcf21 frameshift allele (a,c), and trans-heterozygous for different frameshift alleles (b,d). (e–h) Internally V5 epitope tagged Tcf21 and Tbx18 proteins are detectable by anti-V5 antibodies. Comparison between protein expression as detected by immunohistochemistry using anti-V5 antibodies (e,g) and mRNA expression detected by whole mount in situ hybridization (f,h).
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Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.
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