FIGURE SUMMARY
Title

4D holographic microscopy of zebrafish larvae microcirculation

Authors
Donnarumma, D., Brodoline, A., Alexandre, D., Gross, M.
Source
Full text @ Opt. Express

(a, b) Zoom of the upper right hand corner of the intensity images |𝐻̃4𝜑(𝑘𝑥,𝑘𝑦)|2 obtained with 4-phase holograms by reconstructing the MO pupil plane. (c) Fourier space hologram obtained after translation of the selected zone in the center of the calculation grid. The purple and green zones correspond to the holograms related to each illumination direction. The sample is a ground glass (a) or zebrafish sample (b,c).

Reconstructed intensity image of RBCs made with the 2-phase hologram H2φ,m (see Eq. (4)) where the time index is m = 77. The purple and green components Hpurple,m(x,y,z) and Hgreen,m(x,y,z) of the images were obtained by selecting the purple and the green zones of Fig. 2(c). Reconstruction was made with z = −26.7 (a), z = 0 (b) and z = +26.7 μm (c). The displayed images (a..c) correspond to image 77 of file Visualization 1, Visualization 2 and Visualization 3. Dorsal side left, anterior to the top. Bar is 100 μm.

Averaged intensity reconstructed images made with 2 phases holograms: H2φ,m = Im − Im−1 and with ω1 = ω2. The purple and green components 〈|Hpurple(x,y,z)|2〉 and 〈|Hgreen(x,y,z)|2〉 are calculated with Eq. (8). Reconstruction was made with z = 0 (a), z = +26.7 (b) and z = +53.5 μm (c). The displayed images correspond to image 51 (a), 76 (b) and 101 (c) of file Visualization 4. Bar is 100 μm.

Results of the 3D reconstruction made without (a) and with (b) the cleaning algorithm. (a) cuts of 〈|Hpurple(x,y,z)|2〉 and 〈|Hgreen(x,y,z)|2〉 along the planes XY (upper-right image highlighted in blue), YZ (upper-left, red) and XZ (lower, yellow). (b) cuts of 〈|Spurple,θ|2〉 and 〈|Sgreen,θ|2〉 along the planes XY, YZ and XZ. In (a) and (b) the cuts correspond respectively to images 64 of the video file Visualization 5 and Visualization 6. The blue, red and yellow lines represent the relative positions of the planes XY, YZ and XZ.

Instantaneous projections |Spurple,θ|2 and |Sgreen,θ|2. The projections correspond to images 1, 32 and 46 of the video file Visualization 7, i.e. to time m = 1 and angle θ = −30° (a), m = 32 and θ = 0° (b), and m = 46 and θ = +15° (c). The display is made in arbitrary logarithmic scale: |Spurple,θ|2 is displayed in purple, |Sgreen,θ|2 in green. Bar is 100 μm.

Averaged projection 〈|Spurple,θ|2〉 and 〈|Sgreen,θ|2〉. The projections correspond to images: 1, 41 and 61 of the video file Visualization 8 i.e. to time m = 1 and angle θ = −40° (a), m = 1 and θ = 0° (b), and θ = +20° (c). The display is made in arbitrary logarithmic scale: 〈|Spurple,θ|2〉 is displayed in purple, 〉|Sgreen,θ|2〉 in green. Bar is 100 μm.

Averaged projections 〈|Spurple,θ|2〉 and 〈|Sgreen,θ|2〉 superimposed with the instantaneous projections |Spurple,θ|2 and |Sgreen,θ|2. The projections correspond to images 1, 32 and 46 of file Visualization 9, i.e. to time index m = 1 and projection angle θ = −30° (a), m = 32 and θ = 0° (b), m = 46 and θ = +15° (c). The display is made in arbitrary logarithmic scale: 〈|Spurple,θ|2〉 is displayed in purple, 〉|Sgreen,θ|2〉 in green. Bar is 100 μm.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Opt. Express