Radiation enhanced invasion via AEG-1 in a zebrafish model. DiI labelled, stable AEG-1 knockdown and negative control cells of the SW480 cell line were injected into the perivitelline space of 48 h old zebrafish embryos and irradiated with 0-10 Gy. After incubation for 24 h, the numbers of cells posterior to the anal opening were manually counted. A. Dose dependent increase of invaded SW480 cells with a significant invasion after 10 Gy radiation (p=0.014). B. AEG-1 knockdown cells had a significant lower cell invasion compared to the unirradiated negative control cells (p=0.002). Radiation increased the invasion significantly upon 10 Gy radiation in the negative control cells (p=0.007) whereas no increase was seen in the AEG-1 knockdown cells. The total numbers of embryos for each group were at least 30. Data are presented as the average number of cells/embryo for each cell line ± SEM. Only significant p-values (p<0.05) are presented. NC: negative control; AEG-1: AEG-1 knockdown.