(A) In vivo imaging of 4-dpf old live transgenic cyp19a1b-GFP zebrafish larvae exposed to EE2, BPA, and various bisphenol A analogs.For each chemical the concentration used is 1 µM excepted for EE2 (1 nM) and the vehicle (DMSO). Dorsal views of the brains. Anterior is to the top. Bar = 20 µm. (B) Quantification of GFP fluorescence in zebrafish larvae exposed to the various compounds. Results are expressed as fold induction above control. Data re presented as mean ± SEM of 20 specimens per condition (n = 20). a: p < 0.05 vs. DMSO; b: p < 0.05 vs. EE2, c: p < 0.05 vs. BPA, d: p < 0.05 vs. BPS, e: p < 0.05 vs. BPF, f: p < 0.05 vs. AF.

Distribution of cyp19a1b transcripts in 7-dpf old zebrafish brains after treatments with EE2 (A2-K2) and BPA (A3-K3) and compared to the DMSO control (A1-K1). Images of transverse sections through the rostrocaudal axis of brains. Arrowheads highlight areas of labeling. For all images, dorsal is to the top. Scale bar = 50 µm.

Distribution of cyp19a1b transcripts in 7-dpf old zebrafish brains after treatments with EE2 (A2-K2) and BPA (A3-K3) and compared to the DMSO control (A1-K1). Images of transverse sections through the rostrocaudal axis of brains. Arrowheads highlight areas of labeling. For all images, dorsal is to the top. Scale bar = 50 µm.

In situ expression of cyp19a1b at the level of the caudal hypothalamus (7-dpf old larvae) after treatments with various BPA analogs (E-H) and comparison with negative (A,C) and positive (B,D) controls.

Distribution of cyp19a1b transcripts in a 7-dpf old zebrafish brain after treatment with BPAP (A2-H2) and compared to a BPA-treated brain (A1-H1). Images of transverse sections through the rostrocaudal axis of brains. Arrowheads highlight areas of labeling. For all images, dorsal is to the top. Scale bar = 50 µm.