arl13b and sec10 interact synergistically in cilia-related phenotypes. (A–H) Gross phenotypes of zebrafish embryo morphants at 3 d postfertilization (dpf) (A–D) and 6 dpf (E–H), on lateral view, after injection of MOs for arl13b and/or sec10. A synergistic interaction resulting in small eyes (arrow), pericardial edema (asterisk), and tail curvature (arrowhead) was observed upon coinjection of suboptimal doses of 2 ng of arl13b MO plus 7.5 ng of sec10 MO at both 3 and 6 dpf (D and H, respectively), which do not result in an abnormal phenotype when injected alone (B, C, F, and G); n = 535. (I) Histogram showing quantification of the effect of MOs at 3 dpf. Coinjection of suboptimal doses of 2 ng of arl13b MO plus 7.5 ng of sec10 MO results in a significant increase in the percentage of abnormal phenotypes (*p < 0.001) compared with injection of the MO alone (2 ng of arl13b MO or 7.5 ng of sec10 MO). Scale bars, 1 mm.
|Acknowledgments:||ZFIN wishes to thank the journal Molecular biology of the cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Mol. Biol. Cell|