ZFIN All Figures, Caruffo <i>et al.</i>, 2015

Caruffo et al., 2015

ZDB-PUB-151027-5
PMID:26500633
Fig. 2
Fig. 4

FIGURE SUMMARY

Title: Potential probiotic yeasts isolated from the fish gut protect zebrafish (Danio rerio) from a Vibrio anguillarum challenge
Authors: Caruffo, M., Navarrete, N., Salgado, O., Díaz, A., López, P., García, K., Feijóo, C.G., Navarrete, P.
Source: Full text @ Front Microbiol

Fig. 2

Stereoscopic observation of the 5-([4,6-Dichlorotriazin-2-yl] amino) fluorescein hydrochloride (DTAF)-labeled V. anguillarum in zebrafish larvae. (A) 5 dpf wild type (WT) larvae were inoculated by immersion with 10⁷ UFC/ml DTAF stained V. anguillarum: (i) lateral view scheme of the gut; (ii) lateral view of challenged larvae. (B) 5 dpf Tg(Lyz:DsRed)^nz50 larvae inoculated by immersion with 10⁷ UFC/ml DTAF-stained V. anguillarum (challenge) or sterile E3 as a control (control). (C) Quantification of neutrophils migration was performed in the selected area (yellow rectangle).

Fig. 4

Colonization studies and microscopic observation of the yeast–zebrafish interactions. (A) Microscopic observation at 0 and 5 dpi of larvae (n = 60) inoculated by immersion with 5 × 10⁶ UFC/ml DTAF-fluorescent yeast. (B) Viable yeast count (UFC/larvae) of larvae inoculated by immersion with 5 × 10⁶ UFC/ml DTAF-fluorescent yeast.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Microbiol

Caruffo et al., 2015 ZDB-PUB-151027-5 PMID:26500633

Potential probiotic yeasts isolated from the fish gut protect zebrafish (Danio rerio) from a Vibrio anguillarum challenge

Caruffo et al., 2015

ZDB-PUB-151027-5
PMID:26500633