Constitutive activation of protein S6 kinase, 90 kDa, polypeptide 1 (RSK1) accelerates melanoma tumor onset and invasion in zebrafish. A: Melanoma-free survival curve of miniCoopR-green fluorescent protein (GFP)-, miniCoopR-Myr-RSK1-, and miniCoopR-RSK-deltaCCT-injected zebrafish. B: Histologic sections of zebrafish RSK1dCCT- and Myr-RSK1-expressing melanomas were stained with hematoxylin and eosin to assess tumor invasion depth. Arrows indicate muscular invasion by melanoma cells. C: Depth of invasion was measured for each tumor and was quantified by averaging the depth of invasion of four tumors for each group (RSK1dCCT and Myr-RSK1). Data are given as means ± SEM. ***P < 0.001 compared with dominant-negative RSK1-expressing tumors.
|Acknowledgments:||ZFIN wishes to thank the journal The American journal of pathology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Am. J. Pathol.|