Arnold et al., 2015 - Comparative analysis of genes regulated by Dzip1/iguana and Hedgehog in zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists   244(2):211-23 Full text @ Dev. Dyn.

Fig. 1

Summary of genes with significantly altered expression in igu mutants and cyclopamine-treated embryos. A–C: Phenotypes of wild-type (A), igu mutant (B) and cyclopamine-treated wild-type (C) embryos at 48 hpf. Arrows point to hemorrhages. Scale bar = 100 µm. D: Venn diagram of total genes significantly up- or down-regulated in treatment group as compared to their control. The purple circle represents the comparison of igu mutants and wild-type siblings and the blue circle represents the comparison of cyclopamine-treated to DMSO-treated siblings. The overlap represents 40 annotated genes that were changed in both groups. E: Heat map of 40 genes differently expressed in both igu mutant and cyclopamine-treated embryos. Green indicates down-regulation, and red indicates up-regulation. Values on scale bar indicate range of fold changes from 6.2-fold down-regulated to 2.1-fold up-regulated. igu, iguana mutant; cyc, cyclopamine-treated.

PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Long-pec

Fig. 2

In situ hybridization of genes misregulated in igu mutants corresponds to direction of expression change on the microarray. A,A′: Nkx3.2 is absent from the notochord (arrowheads) of igu mutants. B,B′: In floor plate (arrows) and brain (arrowheads), Nkx2.2a signal is decreased in igu mutants. C,C′: Nkx2.2b signal is absent from floor plate (arrows) in igu mutants and decreased in ventral forebrain (arrowheads). D,D′: Nkx2.9 is severely reduced in the brain (arrowheads) and floor plate (arrows) of igu mutants. E,E2: FoxF2a ventral head mesoderm (arrows) signal is reduced in igu mutants compared with wild-type siblings. F,F2: FoxF2b signal in ventral head mesoderm (arrows) of wild-type sibling is greatly reduced in igu mutant. G,G′: Tbx20 transcript is decreased in the branchiomotor neurons (arrows) and heart (arrowheads) in igu mutants. H,H′: Ntn1b is decreased in the head (arrowhead) and floor plate (arrows) of igu mutants. I,I′: Ntn5 is up-regulated in the trunk (arrowheads) of igu mutants. J,J′: Fabp11b is expressed in the eye (arrowhead) and lost in igu mutants. K,K′: Urp2 is expressed in the floor plate (arrowheads) and is absent in igu mutants. L,L′: Pim1 transcript is up-regulated in the brain and eye of igu mutants. M,M2: Shha shows no change between wild-type sibling and igu mutant. N,N2: ptch2 is strongly up-regulated in igu mutants. Scale bars = 100 µm.

Fig. 3

In situ hybridization of genes misregulated in cyclopamine-treated embryos corresponds to direction of expression change on the microarray. A,A2: Nkx3.2 is absent from the jaw joint (black arrowheads), pharyngeal arches (white arrowheads) and notochord (arrow) of cyclopamine-treated embryos. B,B2: Nkx2.2a signal is absent from the floor plate (arrows) and reduced in the brain (arrowheads) of cyclopamine-treated embryos. C,C′,D,D′: Expression of Nkx2.2b (C,C′) and Nkx2.9 (D,D′) is completely lost from brain (arrowheads) and floor plate (arrows) of cyclopamine-treated embryos. E,E′,F,F′: Ventral head expression of Foxf2a (E) and Foxf2b (F) is absent in cyclopamine-treated embryos (E2,F′). G,G2: Tbx20 transcript is decreased in branchiomotor neurons (arrows), but maintains expression in the heart (arrowheads) cyclopamine-treated embryos. H,H′: Ntn1b is decreased in the head (arrowheads) and floor plate (arrows) of cyclopamine-treated embryos. I,I′: Ntn5 is down-regulated in the trunk (arrowheads) of cyclopamine-treated embryos. J,J′: Expression of Fabp11b in the eye (arrowheads) is absent in cyclopamine-treated embryos. K,K′: Urp2 in the floor plate (arrows) is unchanged in cyclopamine-treated embryos. L,L′: Pim1 transcript expression is increased in the brain and eye of cyclopamine-treated embryos. M,M′: Shha shows no change between DMSO and cyclopamine. N,N′: ptch2 is strongly down-regulated in cyclopamine-treated embryos. Scale bars = 100 µm.

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.