FIGURE SUMMARY
Title

Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons

Authors
England, S., Hilinski, W., de Jager, S., Andrzejczuk, L., Campbell, P., Chowdhury, T., Demby, C., Fancher, W., Gong, Y., Lin, C., Machikas, A., Rodriguez-Larrain, G., Roman Rivera, V., and Lewis, K.E.
Source
Fig. 1

Wild-Type. Prim-5 (24 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014. Primer sequences were: Forward: AGCCATTGCAAAACTTCACC. Reverse: AATTAACCCTCACTAAAGGGATGTGCCTTTTCTCATGCAAC. PCR product size: 1168 bp.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: Prim-5
Fig. 2

mindbomb1ta52b mutant. Prim-5 (24 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014. Primer sequences were: Forward: AGCCATTGCAAAACTTCACC. Reverse: AATTAACCCTCACTAAAGGGATGTGCCTTTTCTCATGCAAC. PCR product size: 1168 bp.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: Prim-5
Fig. 3

Wild-Type. Prim-10 (27 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014. Primer sequences were: Forward: AGCCATTGCAAAACTTCACC. Reverse: AATTAACCCTCACTAAAGGGATGTGCCTTTTCTCATGCAAC. PCR product size: 1168 bp.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: Prim-5
Fig. 4

Wild-Type. Prim-10 (27 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014. Primer sequences were: Forward: ACAACATCCAGTGGCTAGGG. Reverse: AATTAACCCTCACTAAAGGGACGGAGACAGGCAGATAAAGC. PCR product size: 653 bp. Not spatially restricted.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: Prim-5
Fig. 5

Wild-Type. Prim-10 (27 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014. Primer sequences were: Forward: CATTCTGATGCTGTAACGACCAC. Reverse: AATTAACCCTCACTAAAGGGAAGCATCCCTTTGAGCTACAGTG. PCR product size: 975 bp.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: Prim-5
Fig. 6

Wild-Type. Prim-10 (27 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014. Primer sequences were: Forward: ATCGAGAGCACACCCGACAAAAG. Reverse: AATTAACCCTCACTAAAGGGACACTAAGTGTTGAAGTGGAAGAGC. PCR product size: 457 bp. Embryos have been over-developed to detect all possible expression.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Prim-5
Fig. 7

Wild-Type. Prim-5 (24 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014. Primer sequences were: Forward: GCGGTTCAAAAGACTTCAGC. Reverse: AATTAACCCTCACTAAAGGGATTCAGTTCTTGCAACAGACG. PCR product size: 897 bp.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Prim-5
Fig. 8

mindbomb1ta52b mutant. Prim-5 (24 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014. Primer sequences were: Forward: GCGGTTCAAAAGACTTCAGC. Reverse: AATTAACCCTCACTAAAGGGATTCAGTTCTTGCAACAGACG. PCR product size: 897 bp.

Fig. 9

Wild-Type. Prim-10 (27 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014. Primer sequences were: Forward: GCGGTTCAAAAGACTTCAGC. Reverse: AATTAACCCTCACTAAAGGGATTCAGTTCTTGCAACAGACG. PCR product size: 897 bp.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Prim-5
Fig. 10

Wild-Type. Prim-10 (27 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014. Primer sequences were: Forward: TTTCTGTCCAACCCCTTCTG. Reverse: AATTAACCCTCACTAAAGGGATTGACCCCACACTGGCTATT. PCR product size: 905 bp.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Prim-5
Fig. 11

Wild-Type. Prim-10 (27 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014. Primer sequences were: Forward: CTGTCTGCAAAGCCACTCAC. Reverse: AATTAACCCTCACTAAAGGGACCTCTGCAGGCTACACATCTC. PCR product size: 641 bp.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Prim-5
Fig. 12

Wild-Type. Prim-5 (24 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014. Primer sequences were: Forward: GGATCAATCAGCAGCAGAGAAAC. Reverse: AATTAACCCTCACTAAAGGGAGGTCATCCTTTACATGCAGAGC. PCR product size: 1055 bp.

Fig. 13

Wild-Type. Prim-10 (27 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014. Primer sequences were: Forward: GGATCAATCAGCAGCAGAGAAAC. Reverse: AATTAACCCTCACTAAAGGGAGGTCATCCTTTACATGCAGAGC. PCR product size: 1055 bp.

Fig. 14

Wild-Type. Prim-10 (27 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014, with the exception that the PCR annealing temperature was increased to 60oC. Primer sequences were: Forward: CCATCCACTCATCACGTGTC. Reverse: AATTAACCCTCACTAAAGGGACTTGCTTGTTAGGCCAGGAG. PCR product size: 873 bp.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Prim-5
Fig. 15

Wild-Type. Prim-5 (24 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014. Primer sequences were: Forward: CAAACGCGCAGACTATTTC. Reverse: AATTAACCCTCACTAAAGGGAGTTGTTGCGTAGGGGACACT. PCR product size: 1043 bp.

Fig. 16

mindbomb1ta52b mutant. Prim-5 (24 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014. Primer sequences were: Forward: CAAACGCGCAGACTATTTC. Reverse: AATTAACCCTCACTAAAGGGAGTTGTTGCGTAGGGGACACT. PCR product size: 1043 bp.

Fig. 17

Wild-Type. Prim-10 (27 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014. Primer sequences were: Forward: CGGCAGTCTTCAGTAAGTTTGA. Reverse: AATTAACCCTCACTAAAGGGATGATGCTGTGTCATCACTGCT. PCR product size: 464 bp.

Fig. 18

Wild-Type. Prim-5 (24 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014. Primer sequences were: Forward: TCCATATCCTCCTCTTCCGACAG. Reverse: AATTAACCCTCACTAAAGGGATGTCATTGGTTCGTTCATCCTCAG. PCR product size: 817 bp.

Fig. 19

Wild-Type. Prim-10 (27 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014. Primer sequences were: Forward: TCCATATCCTCCTCTTCCGACAG. Reverse: AATTAACCCTCACTAAAGGGATGTCATTGGTTCGTTCATCCTCAG. PCR product size: 817 bp.

Fig. 20

Wild-Type. Prim-10 (27 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014. Primer sequences were: Forward: AGCTCATGCGTCTGGATAAC. Reverse: AATTAACCCTCACTAAAGGGAAAACGCCATTACCATTCAGG. PCR product size: 904 bp.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Prim-5
Fig. 21

Wild-Type. Prim-10 (27 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014. Primer sequences were: Forward: CGACGAAAACGAAGAGAAGG. Reverse: AATTAACCCTCACTAAAGGGAGTCCTCGAGCATCTGAAAGG. PCR product size: 925 bp.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Prim-5
Fig. 22

Wild-Type. Prim-5 (24 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014. Primer sequences were: Forward: GACTTCTGGCCTTGTTGAAGTG. Reverse: AATTAACCCTCACTAAAGGGATTTTCTGTCAAGGTTGTCAAGTC. PCR product size: 834 bp.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Prim-5
Fig. 23

Wild-Type. Prim-10 (27 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014. Primer sequences were: Forward: GACTTCTGGCCTTGTTGAAGTG. Reverse: AATTAACCCTCACTAAAGGGATTTTCTGTCAAGGTTGTCAAGTC. PCR product size: 834 bp.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Prim-5
Fig. 24

Wild-Type. Prim-15 (30 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014. Primer sequences were: Forward: GACTTCTGGCCTTGTTGAAGTG. Reverse: AATTAACCCTCACTAAAGGGATTTTCTGTCAAGGTTGTCAAGTC. PCR product size: 834 bp.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Prim-15
Fig. 25

Wild-Type. Prim-25 (36 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014. Primer sequences were: Forward: GACTTCTGGCCTTGTTGAAGTG. Reverse: AATTAACCCTCACTAAAGGGATTTTCTGTCAAGGTTGTCAAGTC. PCR product size: 834 bp.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Prim-25
Fig. 26

Wild-Type. Prim-5 (24 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014. Primer sequences were: Forward: ACACAGAACCCGTCCAGAAC. Reverse: AATTAACCCTCACTAAAGGGAGCGCTTTTAACCTCCAGATG. PCR product size: 883 bp.

Fig. 27

mindbomb1ta52b mutant. Prim-5 (24 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014. Primer sequences were: Forward: ACACAGAACCCGTCCAGAAC. Reverse: AATTAACCCTCACTAAAGGGAGCGCTTTTAACCTCCAGATG. PCR product size: 883 bp.

Fig. 28

Wild-Type. Prim-10 (27 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014. Primer sequences were: Forward: ACACAGAACCCGTCCAGAAC. Reverse: AATTAACCCTCACTAAAGGGAGCGCTTTTAACCTCCAGATG. PCR product size: 883 bp.

Fig. 29

Wild-Type. Prim-10 (27 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014, with the exception that in situ hybridization was performed at 65oC. Primer sequences were: Forward: TGTGGCAAAAGATTGTCAGG. Reverse: AATTAACCCTCACTAAAGGGACGTGCAACAAAGAGGTTTGA. PCR product size: 1019 bp.

Fig. 30

Wild-Type. Prim-10 (27 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014. Primer sequences were: Forward: ACATTGCTGGCAACCCTTAC. Reverse: aattaaccctcactaaagggaTCTTCCAGTGCCCAGTCTCT. PCR product size: 832 bp.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Prim-5
Fig. 31

Wild-Type. Prim-5 (24 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014. Primer sequences were: Forward: GTCAGTGGACGTGTGGTCAG. Reverse: AATTAACCCTCACTAAAGGGAAGCAAAAGGTGACACATCCA. PCR product size: 811 bp. No expression in the hindbrain. No expression in the spinal cord.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: Prim-5
Fig. 32

mindbomb1ta52b mutant. Prim-5 (24 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014. Primer sequences were: Forward: GTCAGTGGACGTGTGGTCAG. Reverse: AATTAACCCTCACTAAAGGGAAGCAAAAGGTGACACATCCA. PCR product size: 811 bp. No expression in the hindbrain.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: Prim-5
Fig. 33

Wild-Type. Prim-10 (27 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014. Primer sequences were: Forward: GTCAGTGGACGTGTGGTCAG. Reverse: AATTAACCCTCACTAAAGGGAAGCAAAAGGTGACACATCCA. PCR product size: 811 bp.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: Prim-5
Fig. 34

Wild-Type. Prim-10 (27 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014. Primer sequences were: Forward: TGAATTTGCTGCTTCACAGG. Reverse: AATTAACCCTCACTAAAGGGACTCGAGAGTGGACCACCATTT. PCR product size: 1082 bp.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Prim-5
Fig. 35

Wild-Type. Prim-15 (30 hpf). Probe synthesis and in situ hybridisation was performed as described in England et al., Identifying Transcription Factors Expressed by Ventral Spinal Cord Interneurons. ZFIN on-line publication, 2014. Primer sequences were: Forward: TGAATTTGCTGCTTCACAGG. Reverse: AATTAACCCTCACTAAAGGGACTCGAGAGTGGACCACCATTT. PCR product size: 1082 bp.

Acknowledgments
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