Combinatorial Suppression of er71/etsrp, gata2, and scl Promotes Cardiogenic Output in Zebrafish Anterior Lateral Plate Mesoderm
(A–I) In situ hybridization depicts hand2 and fli1a expression in uninjected (A), control (B), and etsrp MO embryos (C; 3 ng), scl MO (E; 12.5 ng), gata2 MO (F; 33.4 ng), gata2 (33.4 ng)+scl (12.5 ng) MOs (G), etsrp (3 ng)+scl (3 ng) MOs (H), etsrp (3 ng)+scl (3 ng)+gata2 (33.4 ng) MOs (I)-injected embryos, and etsrp-/- embryos (D).
(J) Expression of hand2 in gata2 (33.4 ng)+scl (12.5 ng) MOs-injected etsrp+/+ and etsrp+/ embryos. The numbers show the severe phenotype. (A–J) Seven-somite stage, dorsal views, anterior to the left.
(K) Lateral view of flia expression in uninjected, etsrp MO, or etsrp+scl+gata2 MO-injected embryos. Scale bar: 500 µm.
See also Table S4.
|Acknowledgments:||ZFIN wishes to thank the journal Stem Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Stem Cell Reports|