FIGURE SUMMARY
Title

Low-volume toolbox for the discovery of immunosuppressive fungal secondary metabolites

Authors
Berthier, E., Lim, F.Y., Deng, Q., Guo, C.J., Kontoyiannis, D.P., Wang, C.C., Rindy, J., Beebe, D.J., Huttenlocher, A., and Keller, N.P.
Source
Full text @ PLoS Pathog.
Fig. 3

In vivo screening of fungal SMs in a wound-free zebrafish screening model.

(A) Schematic of the zebrafish neutrophil recruitment inhibition assay. (B) Stereomicroscope images of fixed and stained fish showing neutrophil recruitment in the tail fin for 3 compounds each applied at a concentration of 10 μM, compared to the negative control (DMSO). (C) Quantification of the neutrophils recruited in the tail fin of zebrafish (*p-value<0.05, the graph is a representative graph of the 3 repeats performed).
Fig. S1

Live/Dead assay on neutrophils treated with Endocrocin. A. Fluorescent microscopy image of a live/dead stain of neutrophils treated with Endocrocin and a negative control. B. Quantification of the ratio of live and dead neutrophils in the fluorescent images.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Pathog.
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