Semi-quantitative expression of dnmt3 genes in zebrafish adult tissues by RT-PCR. The expression of the dnmt3a related genes (dnmt3a1 and dnmt3a2) was more stable across tissues than dnmt3b genes.

Relative expression of dnmt3a1 (A), dnmt3a2 (B), dnmt3b1(C), dnmt3b2 (D), dnmt3b3 (E) and dnmt3b4 (F) in zebrafish embryos incubated at 23 °C (yellow bars), 27 °C (light green bars) or 31 °C (dark green bars). Transcript levels were determined by qPCR and normalised within each developmental stage, using eef1a and b2m as endogenous reference genes. Error bars indicate the standard error of the mean for each treatment (n = 4). Different superscript letters indicate significant differences with embryonic temperature. Separate analyses were performed for each gene and developmental stage. Abbreviations are BL, blastula; GR, germ-ring; BU, bud; 20S, 20 somites; PH, start of pharyngula and PM, protruding mouth.