Agarose gel electrophoresis image of methyl-DNA immunoprecipitated (MeDIP) bisulfite treated ntla and gstp1. The methylated ntla (a) gene is enriched after immunoprecipitation, whereas the un-methylated gstp1 (b) gene is not enriched.

Confirmation of the tiling array data using bisulfite sequencing PCR. Figure S1: Shows relative increase in methylation of coronin-actin binding protein 2ba in tumor compared with control. Figure S2: Relative decrease in methylation of angiopoietin-like 3 in tumor compared with control. Figure S3: Relative decrease in methylation of insulin-like growth factor binding protein 1b in tumor compared with control. (1, 2 and 3: three independent controls, 4, 5 and 6: three independent tumors).