FIGURE SUMMARY
Title

The Pou5f1/Pou3f-dependent but SoxB-independent regulation of conserved enhancer N2 initiates Sox2 expression during epiblast to neural plate stages in vertebrates

Authors
Iwafuchi-Doi, M., Yoshida, Y., Onichtchouk, D., Leichsenring, M., Driever, W., Takemoto, T., Uchikawa, M., Kamachi, Y., and Kondoh, H.
Source
Full text @ Dev. Biol.
Fig. 6

Pou5f1-dependent activation of sox2 in the blastoderm of zebrafish embryo. A. sox2 in situ hybridization of wild-type (a, b, and c) and MZspgm793 (d, e, and f) embryos at 30% epiboly (a and d; animal pole at top, dorsal at right), 75% epiboly (b and e, animal view) and bud (c and f, anterior view, dorsal at top) stages. Note the absence of sox2 expression in the ANP in the MZspgm793 embryos at bud stage. B. Rescue of sox2 expression in MZspgm793 embryos by pou5f1 mRNA injection (50 pg per embryo) at the 1-cell stage, as indicated by qRT-PCR using the ddCT method. C. Activation of zebrafish enhancer N2 by Pou5f1 in a luciferase reporter assay. D. ChIP assay demonstrating Pou5f1 binding to the enhancer N2 region. Embryos were injected with pou5f1-FLAG mRNA or left non-injected, and ChIP was performed using anti-FLAG antibody. qPCR analysis shows enrichment of the enhancer N2 region sequence (N2) in injected embryos without enrichment of the control region (Rpl5b).

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: 30%-epiboly to Bud
Acknowledgments
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Reprinted from Developmental Biology, 352(2), Iwafuchi-Doi, M., Yoshida, Y., Onichtchouk, D., Leichsenring, M., Driever, W., Takemoto, T., Uchikawa, M., Kamachi, Y., and Kondoh, H., The Pou5f1/Pou3f-dependent but SoxB-independent regulation of conserved enhancer N2 initiates Sox2 expression during epiblast to neural plate stages in vertebrates, 354-366, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.
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