FIGURE SUMMARY
Title

Lef1 controls patterning and proliferation in the posterior lateral line system of zebrafish

Authors
Gamba, L., Cubedo, N., Lutfalla, G., Ghysen, A., and Dambly-Chaudiere, C.
Source
Full text @ Dev. Dyn.

Expression of lef1, cxcr4b, and cxcr7b. A,B: Expression of lef1 at 26 and 18 hours postfertilization (hpf), respectively. Dashes outline the otic vesicle. C,D: Expression of cxcr4b at 26 and 20 hpf, respectively. E,F: Expression of cxcr4b at 26 hpf in lef1-MO and in double lef1-MO, sdf1-MO embryos. G,G": expression of gfp (green) and of rfp (red) in cxcr4b:rfp, cldnb:gfp embryos. G": Merge. H,H": Same as G,G", but in lef1-MO embryos. The intensity of the red and green in panels G and H is linearly proportional to the intensity of fluorescence (see material and methods). I,J: expression of cxcr7b at 26 hpf in lef1-MO and in double lef1-MO, sdf1-MO embryos. Arrows indicate cells expressing cxcr7b in the leading region of the primordium. K,L: Expression of cxcr7b at 20 hpf in control and in lef1-MO embryos. The presumptive placode extends from rhombomere r6, labeled by out-of-focus cxcr7 expression (Cubedo et al.,2009), and the anterior edge of the first somite, indicated by arrowheads. In A, C, E, F, I, J, dots delineate the primordium. In B, D, K and L, the presumptive primordium is not readily detected under Nomarski optics, and therefore was not outlined. MO, morpholino oligonucleotide. Scale bars = 50 μm.

Posterior lateral line (PLL) phenotype due to lef1 loss of function. A,B: Entire PLL pattern visualized by alkaline phosphatase labeling in 3 days postfertilization (dpf) control and lef1-MO embryos, respectively. C,D: Reduced primordia in lef1-MO embryos and after partial primordium ablation, respectively. E,F: Consecutive neuromasts at the end of truncated lines due to lef1 inactivation and primordium ablation, respectively. MO, morpholino oligonucleotide. Scale bars = 50 μm.

Quantification of lef1-MO2 efficiency. A: Topology of lef1 gene structure indicating the positions of the morpholino target, in green, and the primers used for quantification, in red. The gene comprises 10 exons. B: Distribution of normally spliced (680 bp) and of morphant (600 bp) mRNA in different morphant conditions. MO, morpholino oligonucleotide.

Effect of lef1 on cell proliferation. A–D: After a 1-hr pulse of bromodeoxyuridine (BrdU) between 19 and 20 hours postfertilization (hpf), the number of labeled cells is much larger in control (A,B) than in lef1-MO embryos (C,D). E: Mean number of replicating cells in the two conditions. MO, morpholino oligonucleotide. Scale bars = 50 μm.

Acknowledgments
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