GFP expression driven by the 2 kb enpep promoter: Fluorescent GFP image superimposed on bright field image. GFP expression is detectable at 28 hpf in the pronephric duct (A, dorsal view). At 48 hpf (B, lateral view, inset dorsal view) expression is present in both duct (d) and tubules (t). Expression persists at 9 dpf (C) when the tubules are more prominent and form several loops.

GFP expression in kidney tubules and ducts: Cross section of 3 dpf (A, B) and 6 dpf (C, D); Tg(enpep:GFP) larva stained with anti-GPF (green) and anti-Laminin (red) antibodies. GFP expression can be detected in both pronephric tubules (“t”, A, C) and ducts (“d”, B, D) as well as in podocyte like cells (arrowheads) of the glomerulus (“g” A, C). At 6 dpf several loops of the tubules are present (C). No expression is present in the intestine (i), esophagus (e) or liver (l).

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 6

Unillustrated author statements

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Adult
Acknowledgments
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Reprinted from Gene expression patterns : GEP, 11(1-2), Seiler, C., and Pack, M., Transgenic labeling of the zebrafish pronephric duct and tubules using a promoter from the enpep gene, 118-121, Copyright (2011) with permission from Elsevier. Full text @ Gene Expr. Patterns