FIGURE SUMMARY
Title

Characterization of an lhx1a transgenic reporter in zebrafish

Authors
Swanhart, L.M., Takahashi, N., Jackson, R.L., Gibson, G.A., Watkins, S.C., Dawid, I.B., and Hukriede, N.A.
Source
Full text @ Int. J. Dev. Biol.
Fig. 1

Construct design and early Tg(lhx1a:EGFP)pt303 expression.
(A) Schematic of lhx1a:EGFP construct. (B-S) Tg(lhx1a:EGFP)pt303 embryos. (B-G) Tg(lhx1a:EGFP)pt303 expression. In situ hybridization for egfp. (N-S) In situ hybridization for lhx1a. (B,H,N) Shield stage embryos, animal view. (C,I,O) Shield stage embryos, dorsal view. Arrow marks the shield and bracket denotes the marginal cells. (D,J,P) 5-somite stage embryos, dorsal-posterior view. Arrow points to intermediate mesoderm and arrowhead marks the notochord. (E,K,Q) 5-somite stage embryos, lateral view. Anterior is to the left. (F,L,R) 10-somite stage embryos, dorsal-posterior view. Arrow points to the intermediate mesoderm and arrowhead marks the notochord. White arrow denotes anterior expression domain of lhx1a in the intermediate mesoderm. (G,M,S) 10- somite stage embryos, lateral view. Anterior is to the left. Abbreviations: D, diencephalon; NK, neural keel; P, polster.

Fig. 2

Expression of Tg(lhx1a:EGFP)pt303 during later development.
All animals are oriented such that anterior is to the left. (A-P) Tg(lhx1a:EGFP)pt303 embryos and larvae. ( A , B , G , J , M - P ) Tg(lhx1a:EGFP)pt303 expression. (C,D,H,K) In situ hybridization for egfp. (E,F,I,L) In situ hybridization for lhx1a. (A-F) 24 hpf embryos. Arrow marks position of pronephric proximal tubule and arrowhead delineates distal tubule and duct. (A,C,E) Lateral view, bracket denotes hindbrain. (B,D,F) Dorsal view. (G-I) 48 hpf larvae, arrow in H marks distal tubule and duct in the kidney. (J-L) 72 hpf larvae. (J) Montage of two images to display entire larvae. (M-P) Longitudinal optical sections of transgenic animals. (M) 24hpf embryo. (N-P) 48hpf larva. Abbreviations: aLL, anterior lateral line ganglia; FB, forebrain; HB, hindbrain; MB, midbrain; OV, otic vesicle; pLL, posterior lateral line ganglia; SC, spinal column; TB, tailbud.

Fig. 3

Responsiveness of Tg(lhx1a:EGFP)pt303 to perturbations in Nodal signaling.
(A-H) Shield stage Tg(lhx1a:EGFP)pt303 embryos. (A-C,E-G) Animal view. (D,H) Lateral view. (A,E) Uninjected control embryos. (B,F) Embryos injected with 125pg mCherry RNA. (C,D,G,H) Embryos injected with 100pg mCherry RNA and 25pg ndr1 RNA. (A-D) Tg(lhx1a:EGFP)pt303 expression. (B) Overlay of mCherry expression (red) displays an embryo that was successfully injected. (E-H) In situ hybridization for lhx1a.

Fig. 4

Modulation of Tg(lhx1a:EGFP)pt303 expression by SB-431542.
(A-O) Shield stage Tg(lhx1a:EGFP)pt303 embryos, animal view. (A,D,G,J,M) Untreated control embryos. (B,E,H,K,N) Embryos treated with 0.8% DMSO. (C,F,I,L,O) Embryos treated with 800 μM SB-431542. (A-C) Tg(lhx1a:EGFP)pt303 expression. (D-F) In situ hybridization for lhx1a. (G-O) Fluorescent in situ hybridization. (G-I) egfp. (J-L) eve1. (M-O) Merged images.

Acknowledgments
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