StcE induces mislocalization of zebrafish neutrophils.

(A) Zebrafish embryos at 3 dpf were wounded in the ventral fin in the presence of 25 μg/mL StcE, hiStcE, or vehicle control and fixed after 24 hours for whole-mount immunofluorescence with anti-mpo staining. Arrows indicate abnormal localization of neutrophils in the fin of StcE-treated zebrafish. The edge of the fin is outlined in white, and the site of the wound is indicated by an asterisk. The scale bar represents 200 μm. (B) Images from independent experiments were blinded and the number of neutrophils in the fin counted. Data are shown as means of individual experiments (shapes)±S.E.M. and total combined mean (bars); **, p<0.01 of combined means by one-way ANOVA with Dunnett's post test.