Fig. 8 Loss of pronephric tubule tissue in zebrafish tadpoles following transgenic Dkk1 misexpression. Dorsal views of dkk1 wild-type (A) and heterozygous (B and C) transgenic embryos heat-shocked several times starting at 12 h post fertilization (5 somite stage), followed by fixation and staining at 72 h post fertilization with the epithelial pronephric tubule and duct-specific antibody α6F. Insets show enlargements of the pronephric tubule region, with an arrow indicating weak tubule reduction (B, right side), arrowheads indicating strong reduction (C, left and right sides), and an asterisk indicating complete absence (B, left side) of α6F tubule expression. Wild-type embryos exhibit normal α6F staining of pronephric tubules (A). α6F Expression in hsDkk1GFP transgenic embryos was tabulated, with 47.2% of embryos displaying a reduction or complete absence of expression (D).
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Reprinted from Mechanisms of Development, 126(3-4), Lyons, J.P., Miller, R.K., Zhou, X., Weidinger, G., Deroo, T., Denayer, T., Park, J.I., Ji, H., Hong, J.Y., Li, A., Moon, R.T., Jones, E.A., Vleminckx, K., Vize, P.D., and McCrea, P.D., Requirement of Wnt/beta-catenin signaling in pronephric kidney development, 142-159, Copyright (2009) with permission from Elsevier. Full text @ Mech. Dev.