Fig. 8 The expression profile of caspase-8 during embryonic development. RT-PCR analysis of caspase-8 transcripts was performed with total RNA isolated from embryos collected at 1, 6, 18, 36 and 48 h after fertilization (lanes 1-5). Aliquots of the synthesized first-strand cDNAs were amplified with primers for casp8 (Upper) or EF1α as a control (Bottom) and analyzed by agarose-gel electrophoresis. No template DNA and zebrafish casp8 cDNA samples as negative and positive controls were examined in the lanes 6 and 7, respectively.
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Reprinted from Gene, 396(1), Sakata, S.I., Yan, Y., Satou, Y., Momoi, A., Ngo-Hazelett, P., Nozaki, M., Furutani-Seiki, M., Postlethwait, J.H., Yonehara, S., and Sakamaki, K., Conserved function of caspase-8 in apoptosis during bony fish evolution, 134-148, Copyright (2007) with permission from Elsevier. Full text @ Gene